DNA fingerprinting evidence for the phylogenetic relationship of eight species and subspecies of {\sl Dendrolimus} (Lepidoptera: Lasiocampidae) in China-猫眼课题宝

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2004

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Aibing Zhang;X. Kong;Dianmo Li;You-Qiao. Liu

马尾松毛虫、文山松毛虫、松毛虫、松毛虫、油松毛虫、油松毛虫、马尾松毛虫、油松毛虫、油松毛虫、马尾松毛虫、马尾松毛虫等8种松毛虫和松毛虫亚种是近年来油松林中危害最严重、经济危害最大的害虫。初步筛选了20个10-寡核苷酸引物。利用13对引物，共扩增出168个多态性分子标记，对中国松毛虫属8个类群的系统发育关系进行了分析。对松毛虫属8个分类群的NeiS遗传距离(NeiS D)进行了估算。配对S D在0.3780-0.7360的范围内变化。传统上属于马尾松毛虫的两个亚种--德昌毛竹和文山毛竹与马尾松毛虫的遗传距离较近。基于UPGMA的松毛虫属8个种和亚种之间的详细系统发育关系如下：(D.Superans(D.tabulaeformis(D.wenshanensis(D.tehchangensis，D.pot tatus)(D.kikuchii(D.spectabilis，D.houi))。

Eight species and subspecies of Dendrolimus, D. punctatus punctatus (Walker),D. punctatus tehchangensis Tsai et Liu,D. punctatus wenshanensis Tsai et Liu,D. kikuchii Matsumura,D. spectabilis Butler,D. tabulaeformis Tsai et Liu,D. superans (Butler),and D. houi Lajonquiere have been the most serious and economically damaging insect pests in Chinese pine forests in recent years. Twenty 10-oligonucliotide primers were initially screened. We used 13 primers, which produced 168 polymorphic molecular markers to analyze the phylogenetic relationship of the eight taxa of Dendrolimus in China. The pair-wise Neis genetic distances (Neis D) were estimated for the eight taxa of Dendrolimus. The pairwise Neis D varied in the range of 0.3780-0.7360. D. p. tehchangensis Tsai et Liu,and D. p. wenshanensis Tsai et Liu,which are two subspecies of D. punctatus traditionally, have nearer genetic distances with D. p. punctatus. The detailed phylogenetic relationship among the eight species and subspecies of Dendrolimus based on UPGMA should be as the following: (D. superans (D. tabulaeformis (D. wenshanensis (D. tehchangensis, D. punctatus))))(D. kikuchii (D. spectabilis, D. houi)).

DNA fingerprinting evidence for the phylogenetic relationship of eight species and subspecies of {\sl Dendrolimus} (Lepidoptera: Lasiocampidae) in China

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