Detection and genotyping of rubella virus from exanthematous patients suspected of having measles using reverse transcription-PCR.
Detection and genotyping of rubella virus from exanthematous patients suspected of having measles using reverse transcription-PCR.
复制标题
使用逆转录 PCR 对怀疑患有麻疹的出疹患者进行风疹病毒检测和基因分型。
DOI：
10.7883/yoken.67.389
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发表时间：
2014
影响因子：
2.2
通讯作者：
H. Minagawa
中科院分区：
文献类型：
--
作者：
Yoshihiro Yasui;Y. Mori;H. Adachi;Shinichi Kobayashi;T. Yamashita;H. Minagawa
2012年7月至2013年3月，在日本爱知县采集了47名疑似麻疹患者的133份临床标本进行病毒学监测。针对此次风疹疫情，对麻疹病毒(MeV)逆转录(RT)-聚合酶链式反应(RT-PCR)方法进行了改进，实现了风疹病毒(RUBV)的同时检测。结果15例患者30份标本RUBV阳性，3例8份标本MeV阳性。对13例患者标本进行RUBV基因分型，12例为2B，1例为1E。这一结果为仅凭临床表现诊断红斑性疾病的难度以及病毒学诊断的必要性提供了额外的证据，以维持基于病例的监测的准确性。此外，结果表明，改进的RT-PCR方法可以作为常规方法，同时检测疑似由这些病毒引起的红斑性疾病患者的临床标本中的MeV和RUBV。
Between July 2012 and March 2013, a total of 133 clinical specimens from 47 patients suspected of having measles were collected for virological surveillance in Aichi Prefecture, Japan. Facing the rubella epidemic, the reverse transcription (RT)-PCR protocol for measles virus (MeV) was modified to simultaneously detect rubella virus (RUBV) in these clinical specimens. As a result, 30 specimens from 15 patients were positive for RUBV and 8 specimens from 3 patients were positive for MeV. The RUBV genotype analysis for the samples from 13 patients revealed 12 samples as 2B and 1 sample as 1E. The results provided additional evidence for the difficulty in the diagnosis of exanthematous diseases based on clinical manifestations alone and the necessity of virological diagnosis to maintain the accuracy of case-based surveillance. Furthermore, the results indicated that the modified RT-PCR protocol could be useful as a routine procedure to simultaneously detect MeV and RUBV in clinical specimens of patients suspected of having exanthematous disease caused by these viruses.