Assessment of liquid chromatography-tandem mass spectrometry approaches for the analysis of ceftiofur metabolites in poultry muscle
Assessment of liquid chromatography-tandem mass spectrometry approaches for the analysis of ceftiofur metabolites in poultry muscle
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DOI：
10.1080/19440049.2011.635348
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Nielen, Michel W. F.
中科院分区：
文献类型：
--
作者：
Berendsen, Bjorn J. A.;Stolker, Linda (A. ) A. M.;Nielen, Michel W. F.
头孢菌素类抗生素在兽医实践中的使用可能在β-内酰胺耐药菌的发展中发挥重要作用。为了检测头孢菌素类抗生素的标签外使用，需要一种分析方法，除了天然化合物外，还可以检测其活性代谢产物。本文评估了使用LC-MS/MS定量分析头孢噻呋的三种方法的适用性，即（A）头孢噻呋、脱呋喃甲酰头孢噻呋和/或脱呋喃甲酰头孢噻呋半胱氨酸二硫化物的分析，（B）头孢噻呋代谢产物衍生为脱呋喃甲酰头孢噻呋乙酰胺和（C）使用氨进行化学水解，以产生头孢噻呋的标记化合物。我们发现方法A不适用于总头孢噻呋浓度的定量分析或有效检测头孢噻呋的超说明书使用。方法B产生了足够的定量结果，但被认为是单一化合物方法，因为它依赖于硫酯基团的裂解，硫酯基团仅存在于有限数量的头孢菌素抗生素中。方法C显示了足够的定量结果，但与方法B相比，它适用于一系列头孢菌素类抗生素。因此，它适用于作为家禽组织样品中头孢菌素化合物的广泛定量筛查，以指示家禽养殖中头孢菌素的超说明书使用。基于本研究，得出结论，方法C最适合检测一系列头孢菌素类抗生素的超说明书使用。
The use of cephalosporin antibiotics in veterinary practice is likely to play an important role in the development of beta-lactam-resistant bacteria. To detect off-label cephalosporin antibiotic usage, an analytical method is needed that, besides the native compound, also detects their active metabolites. In this paper, the applicability of three approaches for the quantitative analysis of ceftiofur using LC-MS/MS is assessed, viz. (A) analysis of ceftiofur, desfuroylceftiofur and/or desfuroylceftiofur cystein disulfide, (B) derivatisation of ceftiofur metabolites to desfuroylceftiofur acetamide and (C) chemical hydrolysis using ammonia, to produce a marker compound for ceftiofur. We found that approach A was not suited for quantitative analysis of total ceftiofur concentration or for effectively detecting off-label use of ceftiofur. Approach B resulted in adequate quantitative results, but was considered a single compound method because it depends on cleavage of a thioester group, which is present in only a limited number of cephalosporin antibiotics. Approach C showed adequate quantitative results but, in contrast to approach B, it is applicable to a range of cephalosporin antibiotics. Therefore, it is applicable as a broad quantitative screening of cephalosporin compounds in poultry tissue samples to indicate off-label use of cephalosporins in poultry breeding. Based on this study, it was concluded that approach C is the most suitable to detect off-label use of a range of cephalosporin antibiotics.