Possible involvement of group I mGluRs in neuroprotective effect of theanine
Possible involvement of group I mGluRs in neuroprotective effect of theanine
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DOI：
10.1016/j.bbrc.2004.05.143
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发表时间：
2004-07-16
影响因子：
3.1
通讯作者：
Fujimoto, S
中科院分区：
文献类型：
--
作者：
Nagasawa, K;Aoki, H;Fujimoto, S
我们研究了茶氨酸（一种绿色茶成分）神经保护作用的分子机制，使用原代培养的大鼠皮层神经元，重点是I组代谢型谷氨酸受体（mGluRs）。茶氨酸和第一组mGluR激动剂，DHPG，抑制短暂暴露于谷氨酸引起的神经元的延迟性死亡，这种效果的茶氨酸被废除的第一组mGluR拮抗剂。虽然谷氨酸单独给药降低了磷脂酶C（PLC）-β 1和-γ 1，这是连接到组I mGluRs的神经元表达，其表达是等于与茶氨酸共治疗的控制水平。单独用茶氨酸或DHPG处理5-7天导致PLC-β 1和-γ 1的表达增加，并且茶氨酸的作用被I组mGluR拮抗剂完全消除。这些结果表明，I组mGluRs可能通过增加PLC-β 1和-γ 1的表达水平参与了茶氨酸的神经保护作用。(C)2004年爱思唯尔公司All rights reserved.
We investigated the molecular mechanism underlying the neuroprotective effect of theanine, a green tea component, using primary cultured rat cortical neurons, focusing on group I metabotropic glutamate receptors (mGluRs). Theanine and a group I mGluR agonist, DHPG, inhibited the delayed death of neurons caused by brief exposure to glutamate, and this effect of theanine was abolished by group I mGluR antagonists. Although the administration of glutamate alone decreased the neuronal expression of phospholipase C (PLC)-beta1 and -gamma1, which are linked to group I mGluRs, their expression was equal to the control levels on cotreatment with theanine. Treatment with theanine or DHPG alone for 5-7 days resulted in increased expression of PLC-beta1 and -gamma1, and the action of theanine was completely abolished by group I mGluR antagonists. These findings indicate that group I mGluRs might be involved in neuroprotective effect of theanine by increasing the expression levels of PLC-beta1 and -gamma1. (C) 2004 Elsevier Inc. All rights reserved.