Fractionation of a silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity
Fractionation of a silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity
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DOI：
10.1002/app.23740
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发表时间：
2006-10
影响因子：
3
通讯作者：
J. Yeo;Kwang-gill Lee;H. Kweon;S. Woo;S. Han;Sung-Su Kim;M. Demura
中科院分区：
文献类型：
--
作者：
J. Yeo;Kwang-gill Lee;H. Kweon;S. Woo;S. Han;Sung-Su Kim;M. Demura
以桑蚕丝素蛋白为原料，采用蛋白酶水解后的丝素蛋白组分，采用高效液相色谱分离回收系统，研究分子量可控的B对桑蚕丝素蛋白的保护作用。桑蚕丝素蛋白组分对H2 O2损伤神经细胞的影响。首先用回收技术收集分子量小于约1500的三个主要级分。分子量控制的B的最高保护作用。当使用分子量约为1400的级分时，获得了H2 O2损伤的神经元细胞上的桑蚕丝素蛋白组分。丝素蛋白水解物对H_2O_2损伤神经细胞的保护作用可能与酪氨酸、苯丙氨酸等芳香族氨基酸的含量有关。© 2006 Wiley Periodicals，Inc.应用聚合物科学杂志102：772-776，2006
Fractionated components of Bombyx mori silk fibroin, which were hydrolyzed with protease, were prepared with a preparative-recycling high-performance-liquid-chromatography system to evaluate the protective effects of molecular-weight-controlled B. mori silk fibroin components on H2O2-injured neuronal cells. Three major fractions, having molecular weights less than about 1500, were first collected with the recycling techniques. The highest protective effect of the molecular-weight-controlled B. mori silk fibroin components on H2O2-injured neuronal cells was obtained when a fraction with a molecular weight of approximately 1400 was used. This protective effect of the silk fibroin hydrolysate on H2O2-injured neuronal cells was suggested to correlate with the contents of aromatic amino acids such as tyrosine and phenylalanine. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 772–776, 2006