REDUCTION OF CAVEOLIN AND CAVEOLAE IN ONCOGENICALLY TRANSFORMED-CELLS
REDUCTION OF CAVEOLIN AND CAVEOLAE IN ONCOGENICALLY TRANSFORMED-CELLS
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DOI：
10.1073/pnas.92.5.1381
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发表时间：
1995-02-28
影响因子：
11.1
通讯作者：
LISANTI, MP
中科院分区：
文献类型：
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作者：
KOLESKE, AJ;BALTIMORE, D;LISANTI, MP
小泡是瓶状的非网格蛋白包覆的质膜内陷。除了在胞吸和胞吞中已被证实的作用外，小泡还可能调节来自质膜的信号转导。各种致癌基因对NIH 3T3细胞的转化导致小泡蛋白的细胞水平降低，小泡蛋白是小泡蛋白外壳的主要成分。小窝蛋白的减少与这些转化细胞在软琼脂中生长时形成的菌落大小密切相关。电镜显示，这些转化细胞系在形态上没有小泡。这些观察结果表明，小泡的功能改变可能在致癌转化中发挥关键作用，可能是通过破坏转化细胞中的接触抑制。
Caveolae are flask-shaped non-clathrin-coated invaginations of the plasma membrane. In addition to the demonstrated roles for caveolae in potocytosis and transcytosis, caveolae may regulate the transduction of signals from the plasma membrane. Transformation of NIH 3T3 cells by various oncogenes leads to reductions in cellular levels of caveolin, a principal component of the protein coat of caveolae. The reduction in caveolin correlates very well with the size of colonies formed by these transformed cells when groan in soft agar. Electron microscopy reveals that caveolae are morphologically absent from these transformed cell lines. These observations suggest that functional alterations in caveolae may play a critical role in oncogenic transformation, perhaps by disrupting contact inhibition in transformed cells.