Sex pilus specific bacteriophage to drive bacterial population towards antibiotic sensitivity
Sex pilus specific bacteriophage to drive bacterial population towards antibiotic sensitivity
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DOI：
10.1038/s41598-019-48483-9
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发表时间：
2019-08-30
影响因子：
4.6
通讯作者：
Barrow, Paul
中科院分区：
文献类型：
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作者：
Colom, Joan;Batista, Diego;Barrow, Paul
抗菌素耐药性（AMR）现在是一个主要的全球性问题，主要是由于人类和牲畜过度使用抗生素造成的。在一些抗菌素耐药性细菌中，耐药性是由表达性毛的共轭质粒编码的，这种质粒很容易在细菌群体中传播耐药性。本研究的目的是利用性菌特异性（SPS）噬菌体减少AMR质粒的携带。在这里，我们证明了SPS噬菌体可以杀死AMR大肠杆菌，并在体外选择AMR质粒丢失。我们还首次证明了SPS噬菌体既可以阻止AMR肠炎沙门氏菌感染在鸡体内的传播，又可以使细菌群体转向抗生素敏感性。
Antimicrobial resistance (AMR) is now a major global problem largely resulting from the overuse of antibiotics in humans and livestock. In some AMR bacteria, resistance is encoded by conjugative plasmids expressing sex-pili that can readily spread resistance through bacterial populations. The aim of this study was to use sex pilus-specific (SPS) phage to reduce the carriage of AMR plasmids. Here, we demonstrate that SPS phage can kill AMR Escherichia coli and select for AMR plasmid loss in vitro. For the first time, we also demonstrate that SPS phage can both prevent the spread of AMR Salmonella Enteritidis infection in chickens and shift the bacterial population towards antibiotic sensitivity.