Gene cloning, expression and characterization of a novel xylanase from the marine bacterium, Glaciecola mesophila KMM241.
Gene cloning, expression and characterization of a novel xylanase from the marine bacterium, Glaciecola mesophila KMM241.
复制标题
海洋细菌 Glaciecola mesophila KMM241 的新型木聚糖酶的基因克隆、表达和表征
DOI：
10.3390/md11041173
复制
发表时间：
2013-04-08
期刊：
影响因子：
5.4
通讯作者：
Zhang YZ
中科院分区：
文献类型：
--
作者：
Guo B;Li PY;Yue YS;Zhao HL;Dong S;Song XY;Sun CY;Zhang WX;Chen XL;Zhang XY;Zhou BC;Zhang YZ
与陆地木聚糖酶相比，海洋木聚糖酶的研究相对较少。本研究从海洋细菌Glaciecola Mesophila KMM241中克隆了一个新的木聚糖酶基因xynB，并在大肠杆菌中表达。XynB编码糖苷水解酶(GH)家族8的多域木聚糖酶XynB。重组XynB包括一个功能未知的N-末端结构域(NTD)和一个催化结构域，这是GH家族8的木聚糖酶中结构上的新结构。XynB与rXyn8的同源性最高(38%)。重组XynB的最适pH为6~7，最适温度为35℃，不耐高温，耐盐。XynB是一种内切木聚糖酶，需要至少5个糖基才能有效地切割并将木糖和木戊糖水解成木四糖、木三糖和木二糖。NTD在大肠杆菌中表达，并对其功能进行分析。重组NTD对不溶性木聚糖和Avicel具有较高的结合能力，而与壳聚糖和甲壳素的结合能力较弱。由于NTD与公共数据库中已知的任何碳水化合物结合模块(CBM)序列没有明显的同源性，XynB可能含有一种新的CBM。
Marine xylanases are rather less studied compared to terrestrial xylanases. In this study, a new xylanase gene, xynB, was cloned from the marine bacterium, Glaciecola mesophila KMM241, and expressed in Escherichia coli. xynB encodes a multi-domain xylanase XynB of glycoside hydrolase (GH) family 8. The recombinant XynB comprises an N-terminal domain (NTD) with unknown function and a catalytic domain, which is structurally novel among the characterized xylanases of GH family 8. XynB has the highest identity (38%) to rXyn8 among the characterized xylanases. The recombinant XynB showed maximal activity at pH 6–7 and 35 °C. It is thermolabile and salt-tolerant. XynB is an endo-xylanase that demands at least five sugar moieties for effective cleavage and to hydrolyze xylohexaose and xylopentaose into xylotetraose, xylotriose and xylobiose. NTD was expressed in Escherichia coli to analyze its function. The recombinant NTD exhibited a high binding ability to insoluble xylan and avicel and little binding ability to chitosan and chitin. Since the NTD shows no obvious homology to any known carbohydrate-binding module (CBM) sequence in public databases, XynB may contain a new type of CBM.
登录
查看更多内容
影响因子：
1.6
作者：
Ali, MK;Hayashi, H;Ohmiya, K
通讯作者：
Ohmiya, K
影响因子：
4.8
作者：
Collins, T;Meuwis, MA;Gerday, C
通讯作者：
Gerday, C
影响因子：
5
作者：
Liu, Zhuo;Zhao, Xinqing;Bai, Fengwu
通讯作者：
Bai, Fengwu
影响因子：
2.8
作者：
Waeonukul, Rattiya;Pason, Patthra;Ratanakhanokchai, Khanok
通讯作者：
Ratanakhanokchai, Khanok
影响因子：
5
作者：
Guo, Bing;Chen, Xiu-Lan;Zhang, Yu-Zhong
通讯作者：
Zhang, Yu-Zhong