Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome.
Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome.
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核糖相关染色质结构域的鉴定揭示了核仁在A. thaliana基因组3D组织中的作用。
DOI：
10.1016/j.celrep.2016.07.016
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发表时间：
2016-08-09
期刊：
影响因子：
8.8
通讯作者：
Sáez-Vásquez J
中科院分区：
文献类型：
--
作者：
Pontvianne F;Carpentier MC;Durut N;Pavlištová V;Jaške K;Schořová Š;Parrinello H;Rohmer M;Pikaard CS;Fojtová M;Fajkus J;Sáez-Vásquez J
核仁是核糖体RNA （rRNA）基因转录、加工和核糖体生物发生的场所。然而，核仁在细胞中也起着额外的作用。通过荧光活化细胞分选（FACS）分离核仁，通过深度测序鉴定核仁相关染色质结构域（NADs），比较野生型植物和无突变体的核仁蛋白NUCLEOLIN 1 （NUC1）。NADs主要是具有异染色质特征的基因组区域，包括转座元件（te）、亚端粒区和大多数无活性的蛋白质编码基因。然而，NADs也包括活跃的核糖体RNA基因，以及与之相邻的4号染色体的整个短臂。在改变rRNA基因表达和整体核仁结构的nuc1零突变体中，NADs被改变，端粒与核仁的关联减少，端粒变短。总的来说，我们的研究揭示了NUC1和核仁在染色体空间组织和端粒维持中的作用。
The nucleolus is the site of ribosomal RNA (rRNA) gene transcription, rRNA processing and ribosome biogenesis. However, the nucleolus also plays additional roles in the cell. We isolated nucleoli by Fluorescence Activated Cell Sorting (FACS) and identified Nucleolus-Associated Chromatin Domains (NADs) by deep sequencing, comparing wild-type plants and null mutants for the nucleolar protein, NUCLEOLIN 1 (NUC1). NADs are primarily genomic regions with heterochromatic signatures and include transposable elements (TEs), sub-telomeric regions and mostly inactive protein-coding genes. However, NADs also include active ribosomal RNA genes, and the entire short arm of chromosome 4 adjacent to them. In nuc1 null mutants, which alter rRNA gene expression and overall nucleolar structure, NADs are altered, telomere association with the nucleolus is decreased and telomeres become shorter. Collectively, our studies reveal roles for NUC1 and the nucleolus in the spatial organization of chromosomes as well as telomere maintenance.