Dual-Functionalized Covalent Triazine Framework Nanosheets as Hierarchical Nonviral Vectors for Intracellular Gene Delivery
Dual-Functionalized Covalent Triazine Framework Nanosheets as Hierarchical Nonviral Vectors for Intracellular Gene Delivery
复制标题
双功能化共价三嗪框架纳米片作为细胞内基因传递的分层非病毒载体
DOI：
10.1021/acsanm.1c00477
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发表时间：
2021-04-22
影响因子：
5.9
通讯作者：
Fan, Xiaobin
中科院分区：
文献类型：
--
作者：
Cao, Yaqi;Zhang, Junshi;Fan, Xiaobin
基因治疗是治疗多种疾病的革命性生物医学技术，开发安全高效的细胞内基因递送载体对于基因治疗的成功至关重要。在此，我们报告了一种通过剥离和表面化学修饰构建双功能化共价三嗪框架-聚乙二醇-聚乙烯亚胺（CTF-PEG-PEI）纳米片作为非病毒载体的策略。得益于独特的刷状分层结构，CTF-PEG-PEI可以强力凝聚核酸，并在细胞摄取后有效释放核酸货物。 293T 和 HeLa 细胞中的系统基因递送研究表明，与聚乙烯亚胺 (PEI) 相比，CTF-PEG-PEI 具有优异的基因转染性能和更低的细胞毒性，特别是在低剂量时。
Gene therapy is a revolutionary biomedical technique for the treatment of many diseases, and the development of safe and efficient intracellular gene delivery vectors is crucial for successful gene therapy. Herein, we report a strategy to construct dual-functionalized covalent triazine framework-poly(ethylene glycol)-polyethylenimine (CTF-PEG-PEI) nanosheets as nonviral vectors via exfoliation and surface chemistry modification. Benefiting from the unique brushlike hierarchical structure, CTF-PEG-PEI can strongly condense nucleic acids and efficiently release the nucleic acid cargo after cellular uptake. Systematic gene delivery studies in both 293T and HeLa cells demonstrate that CTF-PEG-PEI has superior gene transfection performance and lower cytotoxicity compared with polyethylenimine (PEI), especially at low dosages.