Soil burrowing Muscina angustifrons (Diptera: Muscidae) larvae excrete spores capable of forming mycorrhizae underground
Soil burrowing Muscina angustifrons (Diptera: Muscidae) larvae excrete spores capable of forming mycorrhizae underground
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DOI：
10.1016/j.myc.2018.02.003
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发表时间：
2018-05-01
期刊：
影响因子：
1.4
通讯作者：
Tuno, Nobuko
中科院分区：
文献类型：
--
作者：
Kitabayashi, Keiko;Tuno, Nobuko
麻蝇（双翅目：蝇科）是一种利用多种真菌的真菌，包括外生菌根真菌的真菌。该物种的幼虫以孢子囊（包括孢子）为食，成熟的幼虫离开孢子囊并在土壤表面以下0-6厘米处化蛹。在这项研究中，我们研究了野芽孢杆菌幼虫是否能够运输外生菌根真菌孢子并促进外生菌根在寄主植物根系上的生长。发现成年幼虫水平移动10-20厘米，远离它们的进食地点，并在地下挖洞。这些游荡的幼虫在肠道中保留了外生菌根真菌孢子，这些孢子在迁移到地下化蛹地点后被排出体外。排出的孢子保留萌发和侵染能力，在寄主根部形成外生菌根。在侵染实验中，施用在地下寄主植物根系附近的外生菌根真菌孢子比施用在地表的外生菌根真菌孢子更有效地形成外生菌根，说明M. angustifrons幼虫在地下运输孢子可以促进外生菌根的形成。这些结果表明，长柄霉幼虫是外生菌根真菌的短距离孢子转运体。(C) 2018年由Elsevier B.V.代表日本真菌学学会出版。
Muscina angustifrons (Diptera: Muscidae) is a mycophagous species that exploits a variety of fungi, including ectomycorrhizal fungi. Larvae of this species have been shown to feed on sporocarps (including spores), and full-grown larvae leave sporocarps and pupate 0-6 cm below the soil surface. In this study, we examined whether M. angustifrons larvae are capable of transporting ectomycorrhizal fungal spores and enhancing ectomycorrhiza growth on host-plant roots. Full-grown larvae were found to move horizontally 10-20 cm from their feeding sites and burrow underground. These wandering larvae retained ectomycorrhizal fungal spores in their intestines, which were excreted following relocation to underground pupation sites. Excreted spores retained germination and infection capacities to form ectomycorrhiza on host-plant roots. In the infection experiments, ectomycorrhizal fungal spores applied in the vicinity of underground host-plant roots were more effective in forming ectomycorrhiza than those applied to the ground surface, suggesting that belowground transportation of spores by M. angustifrons larvae could enhance ectomycorrhizal formation. These results suggested that M. angustifrons larvae act as a short-distance spore transporter of ectomycorrhizal fungi. (C) 2018 Published by Elsevier B.V. on behalf of the Mycological Society of Japan.