YEAST USE TRANSLATIONAL CONTROL TO COMPENSATE FOR EXTRA COPIES OF A RIBOSOMAL-PROTEIN GENE
YEAST USE TRANSLATIONAL CONTROL TO COMPENSATE FOR EXTRA COPIES OF A RIBOSOMAL-PROTEIN GENE
复制标题
DOI：
10.1016/0092-8674(82)90151-9
复制
发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
WARNER, JR
中科院分区：
文献类型：
--
作者：
PEARSON, NJ;FRIED, HM;WARNER, JR
核糖体的有效组装需要核糖体RNA和每种核糖体蛋白质的平衡合成。为了建立负责这种平衡合成的机制，通过向酵母中引入包含基因 tcm1（编码核糖体蛋白 L3）的自主复制质粒来改变其中一种成分的基因剂量。通过选择 URA3 基因的表达，将质粒维持在每个细胞 5-10 个拷贝。含有质粒的细胞转录的L3 mRNA是对照细胞的7.5倍，维持的L3 mRNA是对照细胞的3.5倍，并且合成的L3蛋白不超过对照细胞的1.2倍。核糖体蛋白的平衡合成显然是通过调节翻译效率及其 mRNA 的寿命来维持的。
The efficient assembly of ribosomes requires a balanced synthesis of ribosomal RNA and each ribosomal protein. In an attempt to establish the mechanisms responsible for such balanced synthesis the gene dosage for one of the components was altered by introducing into yeast an autonomously replicating plasmid containing the gene tcm1, which codes for ribosomal protein L3. The plasmid is maintained at 5-10 copies per cell by selection for expression of its URA3 gene. The plasmid-containing cells transcribe 7.5 times as much L3 mRNA as control cells, maintain 3.5 times as much L3 mRNA as control cells and synthesize no more than 1.2 times as much L3 protein as control cells. The balanced synthesis of ribosomal proteins is apparently maintained by modulating both the efficiency of translation and the lifetime of their mRNA.