Fast and Accurate Imaging of Lymph Node Metastasis with Multifunctional Near-Infrared Polymer Dots
Fast and Accurate Imaging of Lymph Node Metastasis with Multifunctional Near-Infrared Polymer Dots
复制标题
利用多功能近红外聚合物点对淋巴结转移进行快速准确的成像
DOI：
10.1002/adfm.201707174
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发表时间：
2018-04-18
影响因子：
19
通讯作者：
Xiong, Liqin
中科院分区：
文献类型：
--
作者：
Cao, Fengwen;Guo, Yixiao;Xiong, Liqin
区域淋巴结转移是癌症进展的重要预后指标。对淋巴结转移的快速准确诊断有很大的需求。在这项工作中，叶酸受体靶向的三峰聚合物点被设计用于淋巴结转移的近红外（NIR）/光声（PA）/磁共振（MR）成像。共聚焦显微镜分析和流式细胞术显示，表达叶酸受体的肺粘膜上皮细胞癌NCI-H292对叶酸功能性聚合物点呈阳性。在体内和离体近红外成像结果验证，制备的聚合物点显示快速和高摄取的转移性淋巴结，可以有效地区分转移和正常淋巴结注射后1小时，并在实时成像引导手术有很大的潜力。此外，通过瘤内注射聚合物点通过NIR成像检测来自荷瘤小鼠的十个转移性淋巴结。此外，在体内PA和MR成像证实了增强PA和MR信号的聚合物点在转移性淋巴结，以及扩大的淋巴结在荷瘤小鼠。本研究的结果提供了一种独特的方法，使用三峰聚合物点的快速和准确的诊断淋巴结转移在体内。
Metastasis to regional lymph nodes is a significant prognostic indicator for cancer progression. There is a great demand for rapid and accurate diagnosis of metastasis to the lymph nodes. In this work, folate receptor-targeted trimodal polymer dots are designed for near-infrared (NIR)/photoacoustic (PA)/magnetic resonance (MR) imaging of lymph node metastasis. Confocal microscopic analyses and flow cytometry show that pulmonary mucosa epithelial cell carcinoma NCI-H292 with expression of the folate receptor is positive for folate-functional polymer dots. In vivo and ex vivo NIR imaging results verify that prepared polymer dots show rapid and high uptake in the metastatic lymph nodes, can effectively distinguish metastatic and normal lymph nodes for 1 h postinjection, and have great potential in real-time imaging-guided surgery. Furthermore, ten metastatic lymph nodes from the tumor-bearing mice are detected by NIR imaging via intratumoral injection of polymer dots. Moreover, in vivo PA and MR imaging confirm the enhanced PA and MR signals of polymer dots in the metastatic lymph nodes as well as enlarged lymph nodes in tumor-bearing mice. The results of this study provide a unique approach using trimodal polymer dots for the rapid and precise diagnosis of lymph node metastasis in vivo.