Zinc Finger Protein5 Is Required for the Control of Trichome Initiation by Acting Upstream of Zinc Finger Protein8 in Arabidopsis
Zinc Finger Protein5 Is Required for the Control of Trichome Initiation by Acting Upstream of Zinc Finger Protein8 in Arabidopsis
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在拟南芥中，锌指蛋白 5 是通过作用于锌指蛋白 8 上游来控制毛状体起始所必需的
DOI：
10.1104/pp.111.180281
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发表时间：
2011-10-01
期刊：
影响因子：
7.4
通讯作者：
Gan, Yinbo
中科院分区：
文献类型：
--
作者：
Zhou, Zhongjing;An, Lijun;Gan, Yinbo
拟南芥（Arabidopsis thaliana）毛状体发育是研究细胞发育、细胞分化和细胞周期的模式系统。我们先前的研究表明，拟南芥中的无光泽花序茎（GIS）家族基因GIS、GIS 2和锌指蛋白8（ZFP 8）通过整合赤霉素（GAs）和细胞分裂素信号来控制芽的成熟和表皮细胞的命运。在这里，我们表明，一个新的C2H2锌指蛋白，ZFP 5，通过GA信号在控制毛状体细胞发育中起着重要作用。ZFP 5的过表达导致心皮和其他花序器官上异位毛状体的形成。与野生型植物相比，ZFP 5功能丧失突变体在萼片、茎生叶、旁枝和主花序茎上表现出减少的毛状体数量。更重要的是，发现ZFP 5介导GAs对毛状体起始的调节。这些结果与ZFP 5表达模式和GA对毛状体起始的区域影响一致。分子分析表明ZFP 5在GIS、GIS 2、ZFP 8和关键毛状体起始调节子GLABROUS1（GL1）和GL3的上游起作用。使用类固醇诱导的激活ZFP 5和染色质免疫沉淀实验，我们进一步证明，ZFP 8是ZFP 5在控制表皮细胞分化的直接目标。
Arabidopsis (Arabidopsis thaliana) trichome development is a model system for studying cell development, cell differentiation, and the cell cycle. Our previous studies have shown that the GLABROUS INFLORESCENCE STEMS (GIS) family genes, GIS, GIS2, and ZINC FINGER PROTEIN8 (ZFP8), control shoot maturation and epidermal cell fate by integrating gibberellins (GAs) and cytokinin signaling in Arabidopsis. Here, we show that a new C2H2 zinc finger protein, ZFP5, plays an important role in controlling trichome cell development through GA signaling. Overexpression of ZFP5 results in the formation of ectopic trichomes on carpels and other inflorescence organs. zfp5 loss-of-function mutants exhibit a reduced number of trichomes on sepals, cauline leaves, paraclades, and main inflorescence stems in comparison with wild-type plants. More importantly, it is found that ZFP5 mediates the regulation of trichome initiation by GAs. These results are consistent with ZFP5 expression patterns and the regional influence of GA on trichome initiation. The molecular analyses suggest that ZFP5 functions upstream of GIS, GIS2, ZFP8, and the key trichome initiation regulators GLABROUS1 (GL1) and GL3. Using a steroid-inducible activation of ZFP5 and chromatin immunoprecipitation experiments, we further demonstrate that ZFP8 is the direct target of ZFP5 in controlling epidermal cell differentiation.