SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans.
SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans.
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SLC6家族转运蛋白SNF-10是蛋白酶介导的秀丽隐杆线虫激活的激活所必需的。
DOI：
10.1016/j.ydbio.2014.06.001
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Stanfield, Gillian M.
中科院分区：
文献类型：
--
作者：
Fenker, Kristin E.;Hansen, Angela A.;Chong, Conrad A.;Jud, Molly C.;Duffy, Brittany A.;Norton, J. Paul;Hansen, Jody M.;Stanfield, Gillian M.
精子的活力对于其定向迁移到卵子至关重要。调节运动的获得和调节，以确保精子在需要的时间和地点移动，从而促进生殖成功。这种现象的一个具体例子发生在秀丽隐杆线虫变形虫精子从圆形精子细胞活化为成熟的爬行精子的分化过程中。精子的激活受到冗余途径的调节，在每种性别的特定时间和地点发生。在这里，我们报告了溶质载体 6 (SLC6) 转运蛋白 SNF-10 的鉴定，它是线虫精子响应雄性蛋白酶激活信号而激活的关键调节因子。我们发现 SNF-10 存在于精子中，并且是雄性激活所必需的，但雌雄同体则不需要。 snf-10 和雌雄同体激活因子的缺失使精子对激活完全不敏感。通过体外测定，我们发现 snf-10 突变精子对蛋白酶处理的反应表现出特定的缺陷，但对其他激活剂没有反应。在激活之前，SNF-10 存在于质膜中，它是接收导致亚细胞形态发生的信号的有力候选者。激活后，它显示出细胞体区域的极化定位，这依赖于 Dysferlin FER-1 介导的膜融合。我们对 snf-10 的发现让我们深入了解两性为实现产生功能性精子的共同目标而采用的不同机制，以及如何调节线虫精子的生理学来控制哺乳动物的运动性。
Motility of sperm is crucial for their directed migration to the egg. The acquisition and modulation of motility are regulated to ensure that sperm move when and where needed, thereby promoting reproductive success. One specific example of this phenomenon occurs during differentiation of the amoeboid sperm of C. elegans as they activate from a round spermatid to a mature, crawling spermatozoon. Sperm activation is regulated by redundant pathways to occur at a specific time and place for each sex. Here, we report the identification of the solute carrier 6 (SLC6) transporter protein SNF-10 as a key regulator of C. elegans sperm activation in response to male protease activation signals. We find that SNF-10 is present in sperm and is required for activation by the male but not by the hermaphrodite. Loss of both snf-10 and a hermaphrodite activation factor render sperm completely insensitive to activation. Using in vitro assays, we find that snf-10 mutant sperm show a specific deficit in response to protease treatment but not to other activators. Prior to activation, SNF-10 is present in the plasma membrane, where it represents a strong candidate to receive signals that lead to subcellular morphogenesis. After activation, it shows polarized localization to the cell body region that is dependent on membrane fusions mediated by the dysferlin FER-1. Our discovery of snf-10 offers insight into the mechanisms differentially employed by the two sexes to accomplish the common goal of producing functional sperm, as well as how the physiology of nematode sperm may be regulated to control motility as it is in mammals.
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影响因子：
30.3
作者：
Dierking, Katja;Polanowska, Jolanta;Pujol, Nathalie
通讯作者：
Pujol, Nathalie
影响因子：
0.7
作者：
Darszon, Alberto;Guerrero, Adan;Wood, Christopher D.
通讯作者：
Wood, Christopher D.
影响因子：
4.4
作者：
Farmer, MK;Robbins, MJ;Pangalos, MN
通讯作者：
Pangalos, MN
影响因子：
3.7
作者：
Kumsta C;Hansen M
通讯作者：
Hansen M
影响因子：
64.8
作者：
Chu, Diana S.;Liu, Hongbin;Meyer, Barbara J.
通讯作者：
Meyer, Barbara J.