Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-lymphoma.
Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-lymphoma.
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DOI：
10.1263/jbb.100.112
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发表时间：
2005-07
影响因子：
2.8
通讯作者：
Kouji Tanaka;A. Ito;Takeshi Kobayashi;T. Kawamura;S. Shimada;Kazuhiko Matsumoto;T. Saida;H. Honda
中科院分区：
文献类型：
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作者：
Kouji Tanaka;A. Ito;Takeshi Kobayashi;T. Kawamura;S. Shimada;Kazuhiko Matsumoto;T. Saida;H. Honda
树突状细胞（Dendritic cells，DCs）是一种有效的抗原提呈细胞，在肿瘤免疫反应中起重要作用。使用这些免疫细胞的免疫疗法已成为一种公认的治疗方式。我们以前报道过使用磁性纳米粒子的热疗诱导抗肿瘤免疫，其可以被包括细胞因子在内的佐剂激活。在本研究中，我们研究了热疗联合DC免疫治疗对小鼠EL 4 T淋巴瘤的治疗作用。磁铁矿阳离子脂质体（MCL）具有正表面电荷，并且由于磁滞损耗而在交变磁场（AMF）中产生热量。将MCL注射到C57 BL/6小鼠的EL 4结节中，使其经受AMF 30 min。肿瘤表面的温度达到45 ℃，并通过控制磁场强度来维持。热疗重复两次，间隔24 h。高温后，将未成熟DC直接注射到EL 4结节中。结果，在75%（6/8）的小鼠中观察到肿瘤完全消退，而在仅通过热疗处理的小鼠的情况下，肿瘤完全消退的百分比为12.5%（1/8）。这种新的癌症疗法，我们称之为“热免疫疗法”，可能适用于晚期恶性肿瘤患者。
Dendritic cells (DCs) are potent antigen-presenting cells that play important roles in regulating immune responses in cancer. Immunotherapy using these immunocytes has become an accepted therapeutic modality. We previously reported that hyperthermia using magnetic nanoparticles induces antitumor immunity, which could be activated by adjuvant including cytokines. In the present study, we investigated the therapeutic effects of hyperthermia combined with DC immunotherapy on mouse EL4 T-lymphoma. Magnetite cationic liposomes (MCLs) have a positive surface charge and generate heat in an alternating magnetic field (AMF) due to hysteresis loss. MCLs were injected into an EL4 nodule in C57BL/6 mice, which were subjected to AMF for 30 min. The temperature at the surface of the tumor reached 45 degrees C and was maintained by controlling the magnetic field intensity. Hyperthermia treatment was repeated twice with 24 h intervals. After hyperthermia, immature DCs were directly injected into the EL4 nodule. As a result, complete regression of tumors in 75% (6/8) of the mice was observed, while the percentage of complete regression of tumors was 12.5% (1/8) in the case of mice treated by hyperthermia alone. This novel cancer therapy, which we have termed "heat immunotherapy", may be applicable to patients with advanced malignancies.