Neutralization of BDNF Attenuates the in vitro Protective Effects of Olfactory Ensheathing Cell-Conditioned Medium on Scratch-Insulted Retinal Ganglion Cells
Neutralization of BDNF Attenuates the in vitro Protective Effects of Olfactory Ensheathing Cell-Conditioned Medium on Scratch-Insulted Retinal Ganglion Cells
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BDNF 的中和减弱嗅鞘细胞条件培养基对划痕损伤的视网膜神经节细胞的体外保护作用
DOI：
10.1007/s10571-010-9626-5
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发表时间：
2011-04-01
影响因子：
4
通讯作者：
You, Si-Wei
中科院分区：
文献类型：
--
作者：
Wang, Tao;Cong, Rui;You, Si-Wei
嗅鞘细胞（OECs）移植是修复中枢神经系统损伤的有效方法之一。以往的研究表明，脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）的表达水平升高与嗅鞘细胞对损伤的皮层、脑干神经元以及视网膜神经节细胞（retinal ganglion cells，RGCs）的保护作用有关，但尚无证据证明移植的嗅鞘细胞是否通过分泌BDNF直接保护损伤的中枢神经元。在本研究中，BDNF的中和作用对成人嗅鞘细胞条件培养液（OEC-CM）对划痕损伤的RGCs的神经保护作用进行了研究。结果表明，OEC-CM保护培养的RGCs免受划痕损伤，BDNF中和抗体中和BDNF减弱了培养基的这种神经保护作用。结论：嗅鞘细胞分泌的神经营养因子（包括BDNF）对体外培养的嗅鞘细胞具有保护作用，BDNF在这种保护作用中起主要作用。
Transplantation of olfactory ensheathing cells (OECs) becomes one of the promising strategies in restoring lost functions of injured central nervous system. Elevated level of expressed brain-derived neurotrophic factor (BDNF) was revealed in the previous studies to be related to the protective effects of OECs on injured cortical and brain stem neurons as well as retinal ganglion cells (RGCs), but no evidence has been obtained to demonstrate whether transplanted OECs protect injured central neurons directly by their secreted BDNF. In the present study, the effects of BDNF neutralization on the neuroprotection of adult OEC-conditioned medium (OEC-CM) on scratch-insulted RGCs were examined. The results showed that OEC-CM protected cultured RGCs from scratch insult, and neutralization of BDNF by BDNF neutralizing antibody attenuated such neuroprotection of the medium. It is thus concluded that neurotrophic factors including BDNF secreted by OECs can protect injured OECs in vitro and BDNF plays a major role in such a protection of OECs.