Core-like particles of an enveloped animal virus can self-assemble efficiently on artificial templates
Core-like particles of an enveloped animal virus can self-assemble efficiently on artificial templates
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DOI：
10.1021/nl070860e
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发表时间：
2007-08-01
期刊：
影响因子：
10.8
通讯作者：
Dragnea, Bogdan
中科院分区：
文献类型：
--
作者：
Goicochea, Nancy L.;De, Mrinmoy;Dragnea, Bogdan
甲型病毒是一种动物病毒，由于其高效的体外自组装特性，在药物传递载体、功能成像探针和纳米颗粒传递囊泡等方面具有巨大的生物医学应用前景。然而，由于其复杂的结构，具有包裹基因组的蛋白质衣壳和由脂质和糖蛋白组成的外膜，具有功能病毒外壳但携带人工货物的病毒样颗粒的体外自组装可能是具有挑战性的。制造这种类似甲型病毒的颗粒可能需要两个步骤：第一，围绕人工核心组装衣壳结构，第二，增加膜层。在这里，我们报告了在第一步取得的进展：在功能化的纳米颗粒核心周围高效地自组装甲型病毒衣壳。
Alphaviruses are animal viruses holding great promise for biomedical applications as drug delivery vectors, functional imaging probes, and nanoparticle delivery vesicles because of their efficient in vitro self-assembly properties. However, due to their complex structure, with a protein capsid encapsulating the genome and an outer membrane composed of lipids and glycoproteins, the in-vitro self-assembly of virus-like particles, which have the functional virus coat but carry an artificial cargo, can be challenging. Fabrication of such alphavirus-like particles is likely to require a two-step process: first, the assembly of a capsid structure around an artificial core, second the addition of the membrane layer. Here we report progress made on the first step: the efficient self-assembly of the alphavirus capsid around a functionalized nanoparticle core.