A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost
A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost
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DOI：
10.2144/000114310
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发表时间：
2015-07-01
期刊：
影响因子：
2.7
通讯作者：
Clark, Matthew Derek
中科院分区：
文献类型：
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作者：
Heavens, Darren;Accinelli, Gonzalo Garcia;Clark, Matthew Derek
长配对（LMP）或“跳跃”文库对于产生连续基因组组装和评估结构变异是非常宝贵的。然而，在许多基因组计划中，高质量（低重复率，精确大小）LMP文库的一致产生已被证明是有问题的。输入DNA的长度和数量是可能影响成功的关键问题。在这里，我们将演示如何从以下内容构建涵盖各种跳转大小的12个库：
Long mate pair (LMP) or "jump" libraries are invaluable for producing contiguous genome assemblies and assessing structural variation. However the consistent production of high quality (low duplication rate, accurately sized) LMP libraries has proven problematic in many genome projects. Input DNA length and quantity are key issues that can affect success. Here we demonstrate how 12 libraries covering a wide range of jump sizes can be constructed from