European brown hare syndrome virus: Molecular cloning and sequencing of the genome
European brown hare syndrome virus: Molecular cloning and sequencing of the genome
复制标题
DOI：
10.1099/0022-1317-77-8-1693
复制
发表时间：
1996-08-01
影响因子：
3.8
通讯作者：
Rasschaert, D
中科院分区：
文献类型：
--
作者：
LeGall, G;Huguet, S;Rasschaert, D
欧洲棕兔综合征病毒（EBHSV）是一种与兔出血症病毒（RHDV）相似的杯状病毒，它的基因组全长7442bp，包含两个开放阅读框。在RHDV中，预测5 '大ORF（ORF 1）编码非结构蛋白和衣壳蛋白的多蛋白前体。小ORF（ORF2）编码一个12 kDa的蛋白质，EBHSV和RHDV的核苷酸同源性为71%，变异均匀地分布在整个基因组中。当比较两个EBHSV序列时，可以检测到微小的差异，表明EBHSV可以变化到与RHDV相同的程度。四个切割位点先前确定的RHDV多蛋白在EBHSV中是保守的。这些测序数据清楚地表明，EBHSV和RHDV共享相似的基因组结构，并确认EBHSV和RHDV是杯状病毒科中的两个不同成员。
The genome of the European brown hare syndrome virus (EBHSV), a calicivirus related to rabbit haemorrhagic disease virus (RHDV), was fully sequenced, It was 7442 bases long and contained two ORFs. In RHDV, the 5' large ORF (ORF1) is predicted to encode a polyprotein precursor to the non-structural and capsid proteins. The small ORF (ORF2) encodes a predicted protein of 12 kDa, Alignment of sequences of EBHSV and RHDV showed 71% nucleotide identity; the changes were uniformly scattered over the whole genome. Minor differences could be detected when comparing two EBHSV sequences, indicating that EBHSV could vary to the same extent as RHDV. Four cleavage sites previously identified on the RHDV polyprotein were conserved in EBHSV. These sequencing data clearly show that EBHSV and RHDV share a similar genomic organization and confirm that EBHSV and RHDV are two distinct members within the family Caliciviridae.