Multiplexed analysis of molecular and elemental ions using nanowire transistor sensors
Multiplexed analysis of molecular and elemental ions using nanowire transistor sensors
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DOI：
10.1016/j.snb.2018.05.018
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发表时间：
2018-10
期刊：
影响因子：
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通讯作者：
Xi Chen;Qitao Hu;Si Chen;N. Netzer;Zhenqiang Wang;Shi-Li Zhang;Zhen Zhang
中科院分区：
文献类型：
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作者：
Xi Chen;Qitao Hu;Si Chen;N. Netzer;Zhenqiang Wang;Shi-Li Zhang;Zhen Zhang
展示了一种具有硅纳米线离子敏感场效应晶体管的集成传感器芯片，用于同时选择性地检测单个样品溶液中的分子和元素离子。传感选择性是通过使用定制的混合基质膜（MMM）对传感器表面进行功能化来实现的，该混合基质膜与目标离子的特定离子载体结合在一起。选择一种仿生容器分子，称为金属有机超级容器（MOSC）作为离子载体来检测分子离子亚甲蓝（MB+），而市售的钠离子载体则用于检测元素离子Na+。传感器表现出近能斯特响应，MB+ 的浓度限值为 56.4±1.8mV/dec，低至 ∼1μM 的浓度限值；Na+ 的浓度限值为 57.9±0.7mV/dec，低至 ∼60μM，两者均具有出色的重现性。 MB+传感器上的广泛控制实验确定了 MOSC 分子在实现稳定且可重复的电位响应中的关键作用。此外，MB+特异性传感器对生理样品中常见的干扰元素离子（例如H+、Na+和K+）表现出显着的选择性。尽管目前 Na+ 特异性传感器的特点是对 MB+ 干扰的免疫力不足，但已确定了根本原因并讨论了普遍适用于疏水性分子离子的补救措施。还进行了河水实验来证明我们传感器的功效。
An integrated sensor chip with silicon nanowire ion-sensitive field-effect transistors for simultaneous and selective detection of both molecular and elemental ions in a single sample solution is demonstrated. The sensing selectivity is realized by functionalizing the sensor surface with tailor-made mixed-matrix membranes (MMM) incorporated with specific ionophores for the target ions. A biomimetic container molecule, named metal-organic supercontainer (MOSC), is selected as the ionophore for detection of methylene blue (MB+), a molecular ion, while a commercially available Na-ionophore is used for Na+, an elemental ion. The sensors show a near-Nernstian response with 56.4 ± 1.8 mV/dec down to a concentration limit of ∼1 μM for MB+and 57.9 ± 0.7 mV/dec down to ∼60 μM for Na+, both with excellent reproducibility. Extensive control experiments on the MB+sensor lead to identification of the critical role of the MOSC molecules in achieving a stable and reproducible potentiometric response. Moreover, the MB+-specific sensor shows remarkable selectivity against common interfering elemental ions in physiological samples,e.g., H+, Na+, and K+. Although the Na+-specific sensor is currently characterized by insufficient immunity to the interference by MB+, the root cause is identified and remedies generally applicable for hydrophobic molecular ions are discussed. River water experiments are also conducted to prove the efficacy of our sensors.