BAFF and BAFF-R of peripheral blood and spleen mononuclear cells in idiopathic thrombocytopenic purpura
BAFF and BAFF-R of peripheral blood and spleen mononuclear cells in idiopathic thrombocytopenic purpura
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特发性血小板减少性紫癜外周血和脾脏单个核细胞的BAFF和BAFF-R
DOI：
10.1080/08916930802397848
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发表时间：
2009-01-01
期刊：
影响因子：
3.5
通讯作者：
Yang, Renchi
中科院分区：
文献类型：
--
作者：
Zhou, Zeping;Chen, Zhenping;Yang, Renchi
BAFF（属于 TNF 家族的 B 细胞激活因子）是 B 细胞稳态的重要组成部分，是 B 细胞正常生存和发育所必需的。为了进一步探讨该细胞因子在特发性血小板减少性紫癜（ITP）发病机制中的作用，测定BAFF/BAFF-R（BAFF受体之一）的表达水平，并分析临床参数与BAFF表达水平的相关性。共有 57 名 ITP 患者入组，25 名年龄和性别匹配的健康志愿者作为对照。从 41 名 ITP 患者和 22 名健康志愿者中获取血清，并使用商业人可溶性 BAFF (sBAFF) ELISA 试剂盒进行分析。通过实时定量 PCR 测定外周血 (PB) (n = 42) 和脾细胞 (SP) (n = 8) 单核细胞 (MNC) 的 BAFF 和 BAFF-R mRNA 表达。正常对照的SPMNC来自三名接受脾切除术的遗传性球形红细胞增多症患者。未经治疗的 ITP 患者的血清 BAFF 水平（中位数 1430 pg/ml，范围：534–5787 pg/ml）高于正常对照（中位数 1120 pg/ml，范围：640–2376 pg/ml，p = 0.006）和治疗 ITP 组（中位数 662 pg/ml，范围 267–1265 pg/ml， p = 0.000）。另一方面，接受治疗的 ITP 患者的血清 BAFF 水平低于正常对照 (p = 0.001)。血小板计数和 BAFF 之间存在弱相关性（Pearson 相关系数为 − 0.242）（p = 0.064）。然而，BAFF 水平与血小板相关免疫球蛋白或免疫球蛋白水平不相关。此外，急性和慢性 ITP 之间的血清 BAFF 水平没有统计学差异 (p = 0.841)。 ITP 的 PBMNC 的 BAFF mRNA 表达高于正常对照，但 BAFF-R mRNA 表达不高于正常对照。 ITP患者SPMNC的BAFF mRNA表达与BAFF-R呈正相关，但正常对照和未经治疗的ITP患者的PBMNC中无相关性。 ITP 中 SPMNC 的 BAFF-R mRNA 表达量比 PBMNC 高 15.29 倍。 BAFF 可能有助于 ITP 的自身免疫和疾病发展。然而，必须仔细考虑 BAFF 血清水平作为 ITP 疾病活动性的替代标志物。
BAFF (B-cell activating factor belonging to the TNF family) is an essential component of B-cell homeostasis, and is required for the normal survival and development of B cells. To further explore the role of this cytokine in the pathogenesis of idiopathic thrombocytopenic purpura (ITP), BAFF/BAFF-R (one of receptors of BAFF) expression levels were determined and the correlation between the clinical parameters and the BAFF expression levels was analyzed. A total of 57 patients with ITP were enrolled and 25 age and sex-matched healthy volunteers served as controls. Serum was obtained from 41 patients with ITP and 22 healthy volunteers and was analyzed with a commercial human soluble BAFF (sBAFF) ELISA kit. BAFF and BAFF-R mRNA expression of peripheral blood (PB) (n = 42) and splenocytes (SP) (n = 8) mononuclear cells (MNC) were determined by real-time quantitative PCR. The SPMNC of normal controls came from three hereditary spherocytosis patients who underwent splenectomy. The untreated patients with ITP had higher serum BAFF levels (Median 1430 pg/ml, Range: 534–5787 pg/ml) than those of normal controls (Median 1120 pg/ml, Range: 640–2376 pg/ml, p = 0.006) and treated ITP group (Median 662 pg/ml, Range 267–1265 pg/ml, p = 0.000). On the other hand, serum BAFF levels of treated patients with ITP were lower than those of normal controls (p = 0.001). There was a weak correlation (the Pearson correlation coefficient is − 0.242) between platelet count and BAFF (p = 0.064). However, BAFF levels did not correlate with platelet associated immunoglobulin or immunoglobulin levels. Moreover, the serum BAFF levels were not statistically different between acute and chronic ITP (p = 0.841). PBMNC of ITP had higher BAFF but not BAFF-R mRNA expression than that of normal controls. BAFF mRNA expression of SPMNC had a positive correlation with BAFF-R in ITP patients but not in PBMNC of normal controls and untreated ITP patients. The BAFF-R mRNA expression of SPMNC was shown to be 15.29 times higher than that of PBMNC in ITP. BAFF might contribute to autoimmunity and disease development in ITP. However, BAFF serum level must be carefully considered as a surrogate marker of disease activity in ITP.