Zinc supplementation reduced DNA breaks in Ethiopian women
Zinc supplementation reduced DNA breaks in Ethiopian women
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DOI：
10.1016/j.nutres.2014.10.006
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发表时间：
2015-01-01
影响因子：
4.5
通讯作者：
Stoecker, Barbara J.
中科院分区：
文献类型：
--
作者：
Joray, Maya L.;Yu, Tian-Wei;Stoecker, Barbara J.
锌状态的评估仍然是一个挑战，主要是因为血清/血浆锌可能无法准确反映个人的锌状态。彗星试验是一种灵敏的检测细胞内DNA链断裂的方法，可作为锌状态的功能性生物标志物。我们假设，锌补充对细胞内DNA损伤的影响，可以评估从埃塞俄比亚的实地研究中收集的样本，使用彗星试验。在一项随机安慰剂对照试验中，40名妇女来自报告的每月肉类消费少于一次且植酸盐水平高的村庄，每天接受20 mg硫酸锌或安慰剂锌，持续17天。采用电感耦合等离子体质谱法测定血浆锌浓度。在研究的基线和终点时，将来自全血的细胞包埋在琼脂糖中，染色并染色，然后由对治疗不知情的研究者进行评分。虽然补充锌对血浆锌没有显著影响，但补充锌的妇女的平均（+/-SEM）彗星尾矩测量值从39.7 +/- 2.7下降到30.0 +/- 1.8（P
Assessment of zinc status remains a challenge largely because serum/plasma zinc may not accurately reflect an individual's zinc status. The comet assay, a sensitive method capable of detecting intracellular DNA strand breaks, may serve as a functional biomarker of zinc status. We hypothesized that effects of zinc supplementation on intracellular DNA damage could be assessed from samples collected in field studies in Ethiopia using the comet assay. Forty women, from villages where reported consumption of meat was less than once per month and phytate levels were high, received 20 mg zinc as zinc sulfate or placebo daily for 17 days in a randomized placebo-controlled trial. Plasma zinc concentrations were determined by inductively coupled plasma mass spectrometry. Cells from whole blood at the baseline and end point of the study were embedded in agarose, electrophoresed, and stained before being scored by an investigator blinded to the treatments. Although zinc supplementation did not significantly affect plasma zinc, mean (+/-SEM) comet tail moment measurement of supplemented women decreased from 39.7 +/- 2.7 to 30.0 +/- 1.8 (P