Effect of polyamines on histone-induced DNA compaction of hyperthermophilic archaea
Effect of polyamines on histone-induced DNA compaction of hyperthermophilic archaea
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DOI：
10.1016/s1389-1723(00)88061-5
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发表时间：
2000-01-01
影响因子：
2.8
通讯作者：
Imanaka, T
中科院分区：
文献类型：
--
作者：
Higashibata, H;Fujiwara, S;Imanaka, T
多胺对组蛋白介导的DNA压缩的影响进行了研究，在体外与古菌组蛋白HpkA从Pyrococcus kodakaraensis KOD 1。琼脂糖凝胶迁移率变化实验表明，当混合物在75 ℃以上孵育时，通过加入多胺（腐胺、亚精胺或精胺）或其乙酰化形式（N-乙酰腐胺、N-1-乙酰亚精胺、N-8-乙酰亚精胺或N-1-乙酰精胺），组蛋白结合的DNA（压缩的DNA）进一步压缩。精胺是最有效的压实增强所有测试的多胺。高浓度的钾离子（1.0 M）并不稳定的DNA的压缩形式，即使双链DNA是稳定地保持在高温下，在这种条件下的热变性。多价多胺在高温环境下对嗜热古菌的核小体具有维持功能。
The effect of polyamines on histone-mediated DNA compaction was examined in vitro with archaeal histone HpkA from Pyrococcus kodakaraensis KOD1. An agarose gel mobility-shift experiment indicated that histone-bound DNA (compacted DNA) was further compacted by addition of a polyamine (putrescine, spermidine, or spermine) or its acetylated form (N-acetylputrescine, N-1-acetylspermidine, N-8-acetylspermidine, or N-1-acetylspermine) when the mixture was incubated at above 75 degrees C. Spermine was most effective in compaction enhancement among all the polyamines tested. A high concentration of potassium ion (1.0 M) did not stabilize the compacted form of DNA even though double-stranded DNA was stably maintained against thermal denaturation at elevated temperatures under this condition. It appears likely that multivalent polyamines have a nucleosome maintenance function in hyperthermophilic archaea in high-temperature environments.