An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus)
An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus)
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DOI：
10.1007/s002990050019
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发表时间：
2000-01-01
影响因子：
6.2
通讯作者：
Strauss, SH
中科院分区：
文献类型：
--
作者：
Han, KH;Meilan, R;Strauss, SH
介绍了一种农杆菌介导法转化杂交棉白杨的方法。和Aigeiros Duby)。该协议允许对几个经济上重要的棉杨杂交种(杨树)进行常规转化。&Gray x P.deltoids Bartr.前男友。马什。和黑杨(P.deltoides x P.ngra L.)以前很难改变的东西。以卡那霉素为选择试剂，对11个不同的杂交棉杨树和1个美洲黑杨品种进行了筛选。其他实验表明，生长素预培养和各种细胞分裂素诱导芽器官发生的有效性之间存在很强的相互作用。这些数据还证明了农杆菌EHA105菌株比C58和LBA4404菌株在T-DNA转移中的优势。
We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray x P. deltoides Bartr. ex. Marsh. and P. deltoides x P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.