Characterization of guinea pig anti-progestin receptor antiserum.

Characterization of guinea pig anti-progestin receptor antiserum.
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豚鼠抗孕激素受体抗血清的表征。

DOI:
10.1210/endo-112-1-396
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发表时间:
1983
期刊:
影响因子:
4.8
通讯作者:
P. Feil
P. Feil
中科院分区:
医学2区
文献类型:
--
作者:
P. Feil

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利用来自兔子宫的部分纯化的受体制剂,在豚鼠中产生抗β-内酰胺酶蛋白受体的抗体。该抗体识别胞质和细胞核的白细胞介素受体,但不识别糖皮质激素、雄激素或雌激素受体。此外,该抗体识别正常人子宫内膜以及人乳腺和子宫内膜肿瘤细胞质中的子宫内膜素受体。抗原:通过在高盐蔗糖梯度上沉降系数的变化、通过从DEAEAffi-GelBlue柱中排除以及通过用蛋白A吸附来判断受体与免疫前或免疫血清之间的抗体复合物形成。
Antibody against the progestin receptor was raised in a guinea pig utilizing a partially purified receptor preparation from rabbit uterus. The antibody recognizes the cytosol and nuclear progestin receptor but not glucocorticoid, androgen, or estrogen receptors. In addition, the antibody recognizes the progestin receptor in normal human endometrium as well as that in human breast and endometrial tumor cytosols. Antigen: antibody complex formation between receptor and preimmune or immune serum was judged by shift in sedimentation coefficient on high-salt sucrose gradients, by exclusion from DEAE Affi-Gel Blue columns, and by adsorption with Protein A.
鸡输卵管孕酮受体的两种 8S 形式的表征。
DOI: --
发表时间: 1982
期刊: The Journal of biological chemistry
影响因子: --
作者:
Dougherty,JJ;Toft,DO
通讯作者: Toft,DO