THE EFFECT OF CELL-SIZE AND RECEPTOR DENSITY ON LIGAND-RECEPTOR REACTION-RATE CONSTANTS
THE EFFECT OF CELL-SIZE AND RECEPTOR DENSITY ON LIGAND-RECEPTOR REACTION-RATE CONSTANTS
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DOI：
10.1016/0161-5890(81)90128-0
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
DELISI, C
中科院分区：
文献类型：
--
作者：
DELISI, C
本文提出了一种计算配体与细胞结合受体相互作用的速率常数的方法，即用溶解的分子之间的反应速率常数来计算配体与细胞结合受体相互作用的速率常数。结果表明，对于相同的反应机制和典型的细胞和分子的尺寸，反应的速率和平衡常数的溶液相配体与受体的细胞结合或以其他方式固定，作为一个珠，可以是几个数量级小于或大于它们的值与分散的受体的反应。这些变化与扩散系数差异关系不大，但与受体的大小和密度以及扩散和反应速率常数之间的关系密切相关。主要方程列表，并说明其效用的数据从文献的简要讨论。
A method is developed for calculating rate constants for ligands interacting with cell-bound receptors in terms of rate constants for reactions between solubilized molecules. The results indicate that for identical reaction mechanisms and typical cellular and molecular dimensions, the rate and equilibrium constants for reaction of solution phase ligand with receptors that are cell-bound or otherwise fixed, as on a bead, can be several orders of magnitude smaller or larger than their values for reactions with dispersed receptors. These variations have little to do with diffusion coefficient differences, but are intimately connected to the size and density of receptors, and the relation between diffusive and reactive rate constants. The main equations are tabularized and their utility illustrated by a brief discussion of data from the literature.