Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:phosphorylcholine Cytidylyltransferase (CCT) genes: the homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression.
Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:phosphorylcholine Cytidylyltransferase (CCT) genes: the homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression.
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pD991-AP3 衍生的 T-DNA 标记株系中 CTP：磷酸胆碱胞苷酰转移酶 (CCT) 基因的表观遗传花同源突变：cct1-1 等位基因的同源突变因 cct2 等位基因而增强，并因 CCT1 过表达而减轻。
DOI：
10.1007/s12374-014-0587-y
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
I.
中科院分区：
文献类型：
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作者：
Hayakawa;Y.;Duan;Z.;Yadake;M.;Tsukano;J.;Yamaoka;Y.;Inatsugi;R.;Fujiki;Y. Oikawa;A.;Saito;K. and Nishida;I.
AP 3-HM是一种类似于apetala 3（ap 3）的同源异型突变，它是由含有AP 3基因-448 ~+47区域的pD 991-AP 3拟南芥T-DNA标记株系识别的。在CTP：磷酸胆碱胞苷酰转移酶基因cct 1 - 1和cct 2的相应突变株系中（Inatsugi et al. 2009），cct 1 -1（F4）的一些花和cct 1 -1 cct 2（F3）的许多花显示ap 3-HM，并且cct 1 -1（F5）和cct 1 -1 cct 2（F4）的所有花变得越来越同源异型。相比之下，cct 2花是正常的所有世代测试。这些结果表明，ap 3-HM与ct 1 - 1等位基因连锁，并被ct 2等位基因增强。cct突变体中的ap 3-HM与富集花蕾中的ap 3转录水平呈负相关。亚硫酸氢盐测序显示内源性AP 3启动子区域incct 1 -1（F3; -317 to -2）和cct 1 -1 cct 2（F3; -473 to -2）存在严重的甲基化，但野生型（Wassilevskaja）和cct 2植物没有显示相应的甲基化。通过表达PISTILATA启动子-AP 3构建体，AP 3-HM incct 1 -1 cct 2突变体得到了完全拯救，并且在与过表达CCT 1的突变体cct 1 -2（哥伦比亚）杂交的F1后代中比与野生型杂交的F1后代中得到了更好的缓解。我们讨论了CCT表达和ap 3-HM抑制之间可能的联系。
Theapetala3(ap3)-like homeotic mutation (ap3-HM) is recognized among pD991-AP3-derivedArabidopsis thalianaT-DNA-tagged lines carrying the -448 to +47 region ofAP3in their T-DNA. In the corresponding mutant lines for CTP:phosphorylcholine cytidylyltransferase genes,cct1-1andcct2(Inatsugi et al. 2009), some flowers ofcct1-1(F4) and many flowers ofcct1-1 cct2(F3) showedap3-HM, and all flowers ofcct1-1(F5) andcct1-1 cct2(F4) became increasingly homeotic. In contrast,cct2flowers were normal for all generations tested. These results demonstrated thatap3-HM is linked to thecct1-1allele and is enhanced by thecct2allele. Theap3-HM in cct mutants was inversely correlated withAP3transcript levels in enriched flower buds. Bisulfite sequencing revealed severe methylation within endogenousAP3promoter regions incct1-1(F3; -317 to -2) andcct1-1 cct2(F3; -473 to -2), but wild-type (Wassilevskaja) and cct2 plants showed no corresponding methylation. Theap3-HM incct1-1 cct2mutants was fully rescued by expressing aPISTILLATApromoter–AP3construct, and was better alleviated in the F1offspring of a cross with theCCT1-overexpressing mutantcct1-2(Columbia) than with the wild type. We discuss possible links between expression of CCT and suppression ofap3-HM.