S-[2-(4-azidosalicylamido)ethylthio]-2-thiopyridine: Radioiodinatable, cleavable, photoactivatible cross-linking agent
S-[2-(4-azidosalicylamido)ethylthio]-2-thiopyridine: Radioiodinatable, cleavable, photoactivatible cross-linking agent
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DOI：
10.1021/bc9600168
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发表时间：
1996-05-01
影响因子：
4.7
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Ebright, YW;Chen, Y;Ebright, RH
S -[2 -（4 - 叠氮水杨酰胺基）乙硫基]-2 - 硫代吡啶（AET）包含：一个2 - 硫代吡啶基部分，它可通过一个可裂解的二硫键实现半胱氨酸特异性地结合到蛋白质中；以及一个4 - 叠氮水杨酰胺基部分，它可进行放射性碘标记和光活化交联。与相关化合物S -[2 -[N -[4 -（4 - 叠氮水杨酰胺基）丁基]氨基甲酰基]乙硫基]-2 - 硫代吡啶[APDP；泽彻勒，G.，奥列尼科夫，A.，和特劳特，R.（1992）《生物化学》31，9526]不同，AET在2 - 硫代吡啶基部分和4 - 叠氮水杨酰胺基部分之间含有一个相对较短的连接臂。在先前的一篇论文中，已表明AET可用于位点特异性的蛋白质 - 蛋白质光交联，以鉴定多蛋白复合物中相邻的蛋白质结构域[陈，Y.，埃布赖特，Y.，和埃布赖特，R.（1994）《科学》265，90]。本文描述了AET的合成、放射性碘标记以及结合到蛋白质中的过程。
S-[2-(4-Azidosalicylamido)ethylthio]-2-thiopyridine (AET) contains: a 2-thiopyridyl moiety, which permits cysteine-specific incorporation into protein through a cleavable disulfide bond, and a 4-azidosalicylamido moiety, which permits radioiodination and photoactivatible cross-linking. In contrast to the related compound S-[2-[N-[4-(4-azidosalicylamido)butyl]carbamoyl]ethylthio]-2- thiopyridine [APDP; Zecherle, G., Oleinikov, A., and Traut, R. (1992) Biochemistry 31, 9526], AET contains a relatively short linker arm between the 2-thiopyridyl moiety and the 4-azidosalicylamido moiety. In a previous paper, it was shown that AET could be used in site-specific protein-protein photo-cross-linking to identify nearest-neighbor protein domains within a multiprotein complex [Chen, Y., Ebright, Y., and Ebright, R. (1994) Science 265, 90]. In this paper, the synthesis, radioiodination, and incorporation into pro-rein of AET are described.