Chiral discrimination of amino acid enantiomers based on different interactions with Cu2+
Chiral discrimination of amino acid enantiomers based on different interactions with Cu2+
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基于与 Cu2 不同相互作用的氨基酸对映体的手性判别
DOI：
10.1016/j.snb.2017.06.143
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zou Gang
中科院分区：
文献类型：
--
作者：
Xia Hongyan;Chen Xiaoxiao;Chen Xingqiang;Cheng Junjie;Liu Yingying;Chen Xin;Zhang Qijin;Zou Gang
氨基酸对映体的手性识别可以使我们更好地了解生物体的基本过程，因为对映体可能具有相反的生物学效应。然而，目前已报道的手性识别方法大多是基于手性主体平台与氨基酸对映体之间的不同相互作用，利用非手性主体平台的手性识别探针尚未被探索。本文利用Cu ~（2+）离子与氨基酸对映体之间的配位作用，建立了一种简便、快速、灵敏的非手性氨基酸对映体识别方法。这些配位作用可导致含有相同量的l-氨基酸对映体和Cu ~（2+）的样品的pH值不同。通过引入pH响应荧光团5（6）-羧基荧光素（CF），荧光分光光度计可以很容易地检测到上述pH值的变化，从而可以用来测定各种氨基酸（包括碱性和酸性氨基酸）的对映体。此外，一个更方便的非手性聚丙烯酰胺（PAM）水凝胶含有CF和Cu 2+也已成功地制造区分氨基酸对映体，甚至在设计的浓度范围内的混合物的对映体过量（ee）。
Chiral discrimination of amino acid enantomers could give us a better understanding of the fundamental processes in organisms since enantiomers may have opposite biological effects. However, most of the reported chiral discrimination methods were based on different interaction between the chiral host platform and amino acid enantiomers, the chiral discrimination probes using achiral host platform have not been explored yet. Herein we reported the development of an easy, rapid and sensitive discrimination methodology of amino acid enantiomers using achiral materials, which relied on the different coordination between Cu2+ions and amino acid enantiomers. These coordinations could result in different pH values of samples containing the same amount ofl- ord-amino acid enantiomers and Cu2+. By introduction of pH-responsive fluorophore 5(6)-Carboxyfluorescein (CF), above pH variation could be easily detected through fluorescence (FL) spectrometter, which could be utilized to determine the variety of amino acid enantiomers (including basic and acidic amino acids). Moreover, a more convenient achiral polyacrylamide (PAM) hydrogel containing CF and Cu2+has also been successfully fabricated to discriminate amino acid enantiomers and even the enantiomeric excess (ee) of mixtures at the designed concentration ranges.