Graduated Controlled Atmosphere: A Novel Approach to Increase "Duke" Blueberry Storage Life
Graduated Controlled Atmosphere: A Novel Approach to Increase "Duke" Blueberry Storage Life
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DOI：
10.3389/fpls.2020.00221
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发表时间：
2020-03-17
影响因子：
5.6
通讯作者：
Terry, Leon A.
中科院分区：
文献类型：
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作者：
Falagan, Natalia;Miclo, Tiana;Terry, Leon A.
蓝莓（Vaccinium corymbosum L.）因其促进健康的潜力而受到高度重视，但它们极易腐烂。控制气调（CA）策略减少蓝莓呼吸代谢，减缓衰老。然而，大气的突然变化会引起果实的物理非生物胁迫，对品质产生负面影响。我们提出了一种基于控制分级的创新方法，以缓慢达到最佳气体储存条件，作为标准CA的替代方案。连续两个季节，“杜克”蓝莓经受四种不同的贮藏条件：对照（空气）;标准CA（在整个实验中突然暴露于5 kPa O-2和10 kPa CO2）; GCA 3和GCA 7（分别在3天和7天内逐渐达到5 kPa O-2和10 kPa CO2）。将果实在0 +/-0.5 ℃下储存28天。实时呼吸测量提供了一个深入了解蓝莓的呼吸反应，他们的气体环境。与对照和标准CA水果相比，接受CA（GCA）处理的蓝莓具有较低的稳态呼吸速率。这表明代谢活动减少，对质量和储存寿命延长产生了积极影响。例如，基于减少的腐烂发生率，与对照相比，GCA 3和GCA 7蓝莓的储存寿命延长了25%。冷藏28 d后，GCA果实硬度比对照和CA果实提高27%。GCA 3在整个实验过程中对维持个体糖浓度具有积极作用，并且两种GCA处理都将抗坏血酸含量保持在接近初始值，而在实验结束时对照果实中降低了44%。这项工作提供了一个范式的转变，如何CA可以应用和更好地了解蓝莓的生理和采后行为。
Blueberries (Vaccinium corymbosum L.) are highly valued for their health-promoting potential, yet they are extremely perishable. Controlled atmosphere (CA) strategies reduce blueberry respiratory metabolism, slowing down senescence. However, the sudden change of atmosphere could elicit a physical abiotic stress in the fruit, negatively affecting quality. We propose an innovative approach based on controlled graduation to slowly reach optimum gas storage conditions as an alternative to standard CA. For two consecutive seasons, "Duke" blueberries were subjected to four different storage conditions: control (air); standard CA (sudden exposure to 5 kPa O-2 and 10 kPa CO2 across the experiment); GCA3 and GCA7 (gradually reaching 5 kPa O-2 and 10 kPa CO2 in 3 and 7 days, respectively). Fruit were stored for 28 days at 0 +/- 0.5 degrees C. Real-time respirometry provided an in-depth insight to the respiratory response of blueberries to their gas environment. Blueberries subjected to the graduated application of CA (GCA) treatments had a lower steady-state respiration rate compared to control and standard CA fruit. This indicated a reduction in metabolic activity that positively impacted quality and storage life extension. For example, GCA3 and GCA7 blueberries had a 25% longer storage life when compared to control, based on reduced decay incidence. In addition, GCA fruit were 27% firmer than control and CA fruit after 28 days of cold storage. GCA3 had a positive effect on maintaining individual sugars concentrations throughout the experiment, and both GCA treatments maintained ascorbic acid content close to initial values compared to a decrease of 44% in the control fruit at the end of the experiment. This work provides a paradigm shift in how CA could be applied and a better understanding of blueberry physiology and postharvest behavior.