A TRUNCATED ISOFORM OF HUMAN CCK-B/GASTRIN RECEPTOR GENERATED BY ALTERNATIVE USAGE OF A NOVEL EXON
A TRUNCATED ISOFORM OF HUMAN CCK-B/GASTRIN RECEPTOR GENERATED BY ALTERNATIVE USAGE OF A NOVEL EXON
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DOI：
10.1006/bbrc.1995.1328
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发表时间：
1995-03-08
影响因子：
3.1
通讯作者：
MIYAKE, A
中科院分区：
文献类型：
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作者：
MIYAKE, A
从人胃组织中分离到一种CCK-B/胃泌素受体亚型cDNA。该cDNA与最初克隆的cDNA仅在5 '端区域不同，并编码截短的同种型（Delta CCK-B），其中CCK-B/胃泌素受体的推定的N-末端胞外结构域完全丢失。基因组CCK-B/胃泌素受体DNA的分离显示，该转录本是由一个新的外显子（称为外显子1b）的交替使用产生的。人胃表达Delta CCK-B和完整CCK-B/胃泌素受体（CCK-BR）的转录物，而人胃癌细胞系AGS仅表达Delta CCK-B转录物。用Delta CCK-B cDNA转染COS-7导致I-125-CCK-8结合位点的出现。其配体选择性与CCK-BR不同。这些结果表明CCK-B/胃泌素受体亚型的分子多样性。(C)出版社：Academic Press
An isoform cDNA of CCK-B/gastrin receptor was isolated from human stomach. This cDNA differed from initially cloned cDNA only in the 5'-end region and encoded a truncated isoform (Delta CCK-B) in which the putative N-terminal extracellular domain of the CCK-B/gastrin receptor was completely lost. Isolation of genomic CCK-B/gastrin receptor DNA revealed that this transcript is generated by alternative usage of a novel exon, termed exon 1b. Human stomach expressed both transcripts of Delta CCK-B and entire CCK-B/gastrin receptor (CCK-BR), whereas human stomach cancer cell line AGS exclusively expressed Delta CCK-B transcripts. Transfection of COS-7 with Delta CCK-B cDNA led to the appearance of binding sites for I-125-CCK-8. Its ligand selectivity was different from that of CCK-BR. These results suggest the molecular diversity in CCK-B/gastrin receptor subtypes. (C) 1995 Academic Press, Inc.