Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes.
Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes.
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不同肠致病性大肠杆菌 (EPEC) 和产志贺毒素大肠杆菌 (STEC) 血清型中肠细胞消失 (LEE) 基因座的特征。
DOI：
10.1111/j.1574-6968.1998.tb13078.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
Trabulsi,LR
中科院分区：
文献类型：
--
作者：
Sperandio,V;Kaper,JB;Bortolini,MR;Neves,BC;Keller,R;Trabulsi,LR
肠致病性大肠杆菌（EPEC）和产志贺毒素大肠杆菌（E.大肠杆菌（STEC）由肠上皮细胞消失基因座（LEE）编码。我们研究了LEE在不同EPEC和STEC菌株中的存在和插入位点。在血清型O 119：H6/H−、O 55：H6、O 55：H7、O 142：H6、O 111 ac：H9/H−、O 111 ab：H9/H−中，LEE插入selCas的下游，如先前对EPEC O 127：H6和STEC O 157：H7所述。在血清型O 111 ac：H8/H−和O26：H11/H−中，LEE插入到先前描述的STEC O26：H−中。然而，在血清型O 111 ab：H25的EPEC中，LEE未插入任一位点，表明K12染色体中存在第三个插入位点。我们还从O 111 ac：H−菌株的LEE的右侧和左手克隆了2.3 kb和1.0 kb的片段，并在这些LEE片段上鉴定了额外的插入序列，这表明LEE可能更大，在这些血清型中可能经历了更多的重组事件。
All proteins involved in the attachment and effacement lesion produced by enteropathogenicEscherichia coli(EPEC) and Shiga-toxin producingE. coli(STEC) are encoded by the locus of enterocyte effacement (LEE). We studied the presence and insertion site of the LEE in different EPEC and STEC strains. In serotypes O119:H6/H−, O55:H6, O55:H7, O142:H6, O111ac:H9/H−, O111ab:H9/H−LEE is inserted downstream ofselCas previously described for EPEC O127:H6 and STEC O157:H7. In serotypes O111ac:H8/H−and O26:H11/H−the LEE is inserted inpheUas previously described for STEC O26:H−. However in EPEC from serotype O111ab:H25 the LEE is not inserted in either site suggesting a third insertion site in the K12 chromosome. We also cloned fragments of 2.3 kb and 1.0 kb from the right and left hand sides of the LEE of a O111ac:H−strain and identified additional insertion sequences on these LEE fragments, suggesting that the LEE may be larger and may have undergone more recombination events in these serotypes.