LOCATION OF RDNA IN THE HETEROMERIC MACRONUCLEUS OF CHILODONELLA-STEINI
LOCATION OF RDNA IN THE HETEROMERIC MACRONUCLEUS OF CHILODONELLA-STEINI
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DOI：
10.1016/s0932-4739(11)80177-x
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发表时间：
1990-03-09
影响因子：
2.9
通讯作者：
STEINBRUCK, G
中科院分区：
文献类型：
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作者：
RADZIKOWSKI, S;STEINBRUCK, G
正如之前所表明的，斯氏Chilodonella的异聚大核的染色质由两个特定区域组成，外部区域（orthomere）由晚期复制的电子致密材料组成，内部区域（paramere）由中等电子密度和早期复制组成，其中心部分具有称为内体的电子致密材料的浓度。本文介绍了有关大核分裂后染色质重组的新细节。分裂后，看不到内体。它在姐妹大核分离后的前两个小时内重新出现。使用原位rDNA-DNA杂交表明，斯氏Chilodonella大核中的rDNA位于外区（orthomere）和内体中。由于较早发现大核的 DNA 含量取决于培养条件，作者认为 DNA 定量变化优先由依赖于细胞食物条件的 rDNA 差异复制引起。
As it was shown previously the chromatin of the heteromeric macronucleus of Chilodonella steini consists of two specific zones, the outer one (the orthomere) made up from late replicating electron dense material, and the inner zone (the paramere) of medium electron density and early replicating, with a concentration of electron dense material called the endosome in its central part. New details regarding the reorganization of the chromatin after macronuclear division are presented here. After the division no endosome is visible. It reappears within the first two hours after the separation of sister macronuclei. With the use of in situ rDNA - DNA hybridization it was shown that the rDNA in the macronucleus of Chilodonella steini is located in the outer zone (orthomere) and the endosome. Since it has been found earlier that the DNA content of the macronucleus depends on culture conditions the authors suggest that the quantitative DNA changes are caused preferentially by differential replication of rDNA dependent on the food conditions of the cells.