Image overlay solution based on threshold detection for a compact near infrared fluorescence goggle system.
Image overlay solution based on threshold detection for a compact near infrared fluorescence goggle system.
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紧凑型近红外荧光护目镜系统基于阈值检测的图像叠加解决方案
DOI：
10.1117/1.jbo.20.1.016018
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发表时间：
2015
影响因子：
3.5
通讯作者：
Gruev,Viktor
中科院分区：
文献类型：
--
作者：
Gao,Shengkui;Mondal,SumanB;Zhu,Nan;Liang,RongGuang;Achilefu,Samuel;Gruev,Viktor
近红外（NIR）荧光成像已显示出巨大的潜力，用于各种临床程序，包括术中图像引导。然而，现有的NIR荧光成像系统要么具有较大的占地面积，要么是手持式的，这限制了它们在术中应用中的使用。我们提出了一种紧凑的近红外荧光成像系统（NFIS）的图像叠加解决方案的基础上阈值检测，它可以很容易地集成到一个护目镜显示系统的术中指导。建议NFIS实现紧凑，重量轻，免提操作，高精度叠加，和实时帧速率。此外，微型和超轻的发光二极管跟踪吊舱很容易与近红外荧光成像结合。基于实验评估，所提出的NFIS解决方案在27 fps下具有25 nM的吲哚菁绿色的检测下限，并且实现了小鼠体内的近红外和可见光图像的高度精确的图像叠加。在65 cm的工作距离下，重叠误差限制在2 mm范围内，这对于临床研究和手术使用是高度可靠的。
Near infrared (NIR) fluorescence imaging has shown great potential for various clinical procedures, including intraoperative image guidance. However, existing NIR fluorescence imaging systems either have a large footprint or are handheld, which limits their usage in intraoperative applications. We present a compact NIR fluorescence imaging system (NFIS) with an image overlay solution based on threshold detection, which can be easily integrated with a goggle display system for intraoperative guidance. The proposed NFIS achieves compactness, light weight, hands-free operation, high-precision superimposition, and a real-time frame rate. In addition, the miniature and ultra-lightweight light-emitting diode tracking pod is easy to incorporate with NIR fluorescence imaging. Based on experimental evaluation, the proposed NFIS solution has a lower detection limit of 25 nM of indocyanine green at 27 fps and realizes a highly precise image overlay of NIR and visible images of micein vivo. The overlay error is limited within a 2-mm scale at a 65-cm working distance, which is highly reliable for clinical study and surgical use.