Decreased expression of Neurensin-2 correlates with poor prognosis in hepatocellular carcinoma
Decreased expression of Neurensin-2 correlates with poor prognosis in hepatocellular carcinoma
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DOI：
10.3748/wjg.15.4844
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发表时间：
2009-10-14
影响因子：
4.3
通讯作者：
Xia, Jian-Chuan
中科院分区：
文献类型：
--
作者：
Ma, Hai-Qing;Liang, Xiao-Ting;Xia, Jian-Chuan
目的：目的：探讨肝细胞癌（HCC）中神经氨酸蛋白2（NRSN 2）的表达及其对预后的预测价值.方法：采用免疫组织化学方法检测110例HCC组织中NRSN 2的表达，并进一步用Western blotting方法进行验证.结果：70.9%的HCC组织中NRSN 2表达降低. HCC中NRSN 2表达缺失与肿瘤大小显著相关（P = 0.006）。肿瘤体积越大，NRSN 2表达越弱。NRSN2阴性表达患者的总生存期显著短于阳性表达患者（P = 0.008）。多因素考克斯回归分析显示NRSN 2表达水平是影响生存的独立因素（P = 0.013）。Western blotting分析进一步证实NRSN 2在肿瘤组织中的表达低于非肿瘤组织。结论：我们的研究表明NRSN 2可能是肝癌的抑癌基因，也是肝癌长期生存的候选生物标志物。(C)2009年，WIG出版社和百世登。All rights reserved.
AIM: To investigate the expression of Neurensin-2 (NRSN2) in hepatocellular carcinoma (HCC) and its prognostic values in predicting survival.METHODS: The expression and prognostic significance of NRSN2 in HCC was examined by performing immunohistochemical analysis using a total of 110 HCC clinical tissue samples, and Western blotting analysis to further confirm the result.RESULTS: Decreased NRSN2 expression was shown in 70.9% cases. Loss of NRSN2 expression in HCC was significantly related to tumor size (P = 0.006). Larger tumor size was related to negative expression of NRSN2. Patients showing negative NRSN2 expression had a significantly shorter overall survival than those with positive expression (P = 0.008). Multivariate Cox regression analysis indicated that NRSN2 expression level was an independent factor of survival (P = 0.013). Western blotting analysis further confirmed decreased expression of NRSN2 in tumor tissues compared with non-tumorous tissues.CONCLUSION: Our study indicated that NRSN2 could be a tumor suppressor gene for HCC and a candidate biomarker for long-term survival in HCC. (C) 2009 The WIG Press and Baishideng. All rights reserved.