Graphene-modified electrodes for sensing doxorubicin hydrochloride in human plasma
Graphene-modified electrodes for sensing doxorubicin hydrochloride in human plasma
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DOI：
10.1007/s00216-019-01611-w
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发表时间：
2019-03-01
影响因子：
4.3
通讯作者：
Szunerits, Sabine
中科院分区：
文献类型：
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作者：
Chekin, Fereshteh;Myshin, Vladyslav;Szunerits, Sabine
阿霉素（DOX）是一种蒽环类分子，是目前临床应用最广泛的抗癌药物之一。由于药物的毒性，已知对DOX患者的系统治疗伴随着一些令人不快的副作用。因此，监测血清样品中的DOX浓度对于避免副作用和确保治疗效率变得越来越重要。在本研究中，我们讨论了用于直接监测临床血液样本中DOX的一次性电化学传感器的构建。该传感器基于在聚酰亚胺片上采用光刻技术形成的柔性集成电极结构中涂覆金电极，并将氮掺杂的还原氧化石墨烯（N-rGO）悬浮在壳聚糖中。在优化条件下，氧化峰电流与DOX浓度在0.010 ~ 15m范围内呈线性关系，检测限为10nM。该传感器适用于监测接受抗癌治疗的患者血清样品中的DOX。
Doxorubicin (DOX), an anthracycline molecule, is currently one of the most widely used anticancer drugs in clinics. Systematic treatment of patients with DOX is known to be accompanied by several unpleasant side effects due to the toxicity of the drug. Thus, monitoring of DOX concentration in serum samples has become increasingly important to avoid side effects and ensure therapeutic efficiency. In this study, we discuss the construction of a disposable electrochemical sensor for the direct monitoring of DOX in clinical blood samples. The sensor is based on coating a gold electrode in a flexible integrated electrode construct formed on polyimide sheets using photolithography, with nitrogen-doped reduced graphene oxide (N-rGO) suspended in chitosan. Under optimized conditions, a linear relationship between the oxidative peak current and the concentration of DOX in the range of 0.010-15M with a detection limit of 10nM could be achieved. The sensor was adapted to monitor DOX in serum samples of patients under anticancer treatment.