Hyperfixation of copper-62-PTSM in rat brain after transient global ischemia.
Hyperfixation of copper-62-PTSM in rat brain after transient global ischemia.
复制标题
短暂性整体缺血后大鼠脑中铜-62-PTSM 的过度固定。
DOI：
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发表时间：
1997
影响因子：
9.3
通讯作者：
A. Yokoyama
中科院分区：
文献类型：
--
作者：
H. Taniuchi;Y. Fujibayashi;Y. Yonekura;J. Konishi;A. Yokoyama
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我们研究了一种潜在的PET灌注剂--62Cu-丙酮醛双(N4-甲基氨基硫脲)(62Cu-PTSM)在大鼠脑内的区域分布，并观察了其在短暂性全脑缺血中的超固定作用。
方法
以~(123)I标记的对碘苯基-N-异丙基甲基苯丙胺(~(123)I-IMP)为参比血流标志物，观察~(62)Cu-PTSM的分布。在Wistar大鼠四支血管阻断30分钟后再循环10分钟、1小时、1天、3天或5天后，测定脑内这两种示踪剂的摄取。大鼠断头前10min静脉注射示踪剂。测定线粒体复合体I和复合体I-III的活性及巯基浓度。
结果
与123I-IMP相比，铜-62-PTSM在再灌流后1小时和1天在脑内的蓄积明显加快(p<；0.01)，这种增强可能是由于超固定作用所致。在这几个时间点，SH浓度显著降低(p<；0.01)。而复合体I的活性不受缺血/再灌流的影响，而复合体I-III的活性降低到对照水平的65-70%(p<；0.01)。
结论
铜-62-PTSM表现出超固定作用，很可能是由于线粒体电子传递障碍导致NADH浓度增加所致。
UNLABELLED
We evaluated the regional distribution of 62Cu-pyruvaldehyde bis(N4-methylthiosemicarbazone) (62Cu-PTSM), a potential PET perfusion agent, in the rat brain and observed hyperfixation in transient global ischemia in rats.
METHODS
The distribution of 62Cu-PTSM was examined in comparison with that of 123I-labeled p-iodophenyl-N-isopropylmethanphetamine (123I-IMP) as a reference blood flow marker. Brain uptake of these two tracers was measured in Wistar rats subjected to 30-min four-vessel occlusion followed by recirculation for 10 min, 1 hr or 1, 3 or 5 days. Tracers were injected intravenously into rats 10 min before decapitation. The activities of Complex I and Complex I-III of mitochondria and the concentration of sulfhydryl (SH) groups were also measured.
RESULTS
Copper-62-PTSM showed accelerated accumulation in the brain at 1 hr and 1 day after reperfusion when compared with that of 123I-IMP (p < 0.01), and this enhancement was considered to be due to hyperfixation. At these time points, SH concentration was significantly decreased (p < 0.01). On the other hand, the activity of Complex I was not influenced by ischemia/reperfusion, but that of Complex I-III was decreased to 65-70% of the control level (p < 0.01).
CONCLUSIONS
Copper-62-PTSM showed hyperfixation most possibly as a result of increased NADH concentration, caused by disturbed electron transport in mitochondria.
铜 62 标记的丙酮醛双（N4-甲硫基氨基脲）铜（II）：合成和评估作为脑和心肌灌注的正电子发射断层扫描示踪剂。
DOI：
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发表时间：
1990
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Green,MA;Mathias,CJ;Welch,MJ;McGuire,AH;Perry,D;Fernandez-Rubio,F;Perlmutter,JS;Raichle,ME;Bergmann,SR
通讯作者：
Bergmann,SR