SELECTIVE NECROSIS AND TOTAL NECROSIS IN FOCAL CEREBRAL-ISCHEMIA - NEUROPATHOLOGIC OBSERVATIONS ON EXPERIMENTAL MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE MACAQUE MONKEY
SELECTIVE NECROSIS AND TOTAL NECROSIS IN FOCAL CEREBRAL-ISCHEMIA - NEUROPATHOLOGIC OBSERVATIONS ON EXPERIMENTAL MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE MACAQUE MONKEY
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DOI：
10.1097/00005072-198401000-00005
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
MARCOUX, FW
中科院分区：
文献类型：
--
作者：
DEGIROLAMI, U;CROWELL, RM;MARCOUX, FW
通过经眼眶圈套器结扎大脑中动脉（MCA），在 87 只清醒的猴子（猕猴和食蟹猴）中诱导暂时（15 分钟至 24 小时）或永久性局灶性脑缺血。 2周后进行神经病理学评估。病变的大小、位置和组织学在 MCA 闭塞的每个时间段内都不同。大多数经历长期缺血（8小时至永久性）的动物都有单一的融合性梗塞，涉及深部结构，有时涉及皮质结构。这些动物完全坏死，其特征是：白质和灰质不加区别地受累；包含星形胶质细胞和单核细胞的相对尖锐的边缘；液化的内部区域，充满脂肪的巨噬细胞和新形成的血管渗透。经历中度至短期缺血（30分钟至4小时）的动物表现出多发性、不融合的深部梗塞。这些动物具有选择性坏死，其特征是：灰质受累，白质相对较少；边界不清、多发且常为血管周围病变，组织破坏不完全；神经元优先丧失以及反应性星形胶质细胞和小胶质细胞增殖。
Temporary (15 min-24 h) or permanent focal cerebral ischemia was induced in 87 awake monkeys (Macaca mulatta and Macaca fascicularis) by transorbital snare ligation of the middle cerebral artery (MCA). Neuropathological evaluation was done 2 wk later. The size, location and histology of lesions varied within each time-period of MCA occlusion. Most animals that underwent long-term ischemia (8 h to permanent) had a single, confluent infarct involving deep and sometimes cortical structures. These animals had total necrosis characterized by: indiscriminate involvement of white and gray matter; relatively sharp margins containing astrocytic and mononuclear cells; an inner zone of liquefaction, infiltrated with fat-laden macrophages and newly formed blood vessels. Animals that underwent moderate to short-term ischemia (30 min-4 h) showed multiple, non-confluent deep infarcts. These animals had selective necrosis characterized by: involvement of gray matter and relative sparing of white matter; poorly circumscribed, multiple and often perivascular lesions with incomplete tissue destruction; preferential loss of neurons and proliferation of reactive astrocytes and microglia.