Persistence of the rotavirus A genome in mesenteric lymph nodes of cattle raised on farms.
Persistence of the rotavirus A genome in mesenteric lymph nodes of cattle raised on farms.
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轮状病毒 A 基因组在农场饲养的牛的肠系膜淋巴结中持续存在。
DOI：
10.1099/vir.0.000191
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Mitake;H.;Ito;N.;Okadera;K.;Okada;K.;Nakagawa;K.;Tanaka;T.;Katsuragi;K.;Kasahara;K.;Nihongi;T.;Sakurai;S.;Tsunemitsu;H. and Sugiyama;M.
以往的研究表明，轮状病毒A（RVA）不仅存在于小肠，而且还存在于各种器官中。据报告，在实验模型中，RVA在肠系膜淋巴结（MLN）中持续存在。然而，目前还没有针对自然条件下动物MLN中RVA的报道。为了研究自然条件下牛MLN中RVA基因组的持久性，本研究采用逆转录-半巢式PCR方法检测了17头经尸检的小牛MLN中的RVA基因组。在17头尸检小牛中的10头（约60%）MLN中检测到RVA基因组。 还检查了来自170头已屠宰的健康成年牛的MLN; 170头牛中的15头（约10%）在其MLN中具有RVA基因组，表明在自然条件下在牛的MLN中经常发现RNA基因组。 遗传分析表明，在MLN的RVAs被归类为G和/或P基因型普遍存在于牛。基本上，肠内容物中的菌株与个体牛MLN中的菌株在遗传上相同，这表明牛RVAs具有从肠道传播到MLN的能力。此外，在RVA阳性牛中，10头尸检小牛中有6头和15头健康成年牛中有12头肠道内容物中的病毒呈阴性，表明牛RVA基因组在消化道中清除病毒后可持续存在于MLN中。
Previous studies revealed that rotavirus A (RVA) is present in not only the small intestine but also various organs. It was reported that RVA persisted in mesenteric lymph nodes (MLNs) in experimental models. However, there have been no reports focused on RVA in MLNs of animals under natural conditions. In this study, in order to investigate the persistence of the RVA genome in MLNs in cattle under natural conditions, reverse transcription-semi-nested PCR was carried out to detect RVA genomes in the MLNs from 17 calves that had been subjected to autopsy examinations. RVA genomes were detected in MLNs from 10 (˜60 %) of the 17 autopsied calves. MLNs from 170 healthy adult cattle that had been slaughtered were also examined; 15 (∼10 %) of the 170 cattle had RVA genomes in their MLNs, indicating that RNA genomes are found frequently in MLNs of cattle under natural conditions. Genetic analyses revealed that RVAs in MLNs were classified as G and/or P genotypes generally prevalent in bovines. Basically, the strains in intestinal contents were genetically identical to those in MLNs from individual cattle, suggesting that bovine RVAs have the ability to spread from the intestine to MLNs. Furthermore, amongst RVA-positive cattle, six of 10 autopsied calves and 12 of 15 healthy adult cattle were negative for the virus in the intestinal contents, indicating that bovine RVA genomes can persist in MLNs after viral clearance in the digestive tract.