Diurnal rhythm of protein carbonyl as an indicator of oxidative damage in Drosophila melanogaster:: Influence of clock gene alleles and deficiencies in the formation of free-radical scavengers
Diurnal rhythm of protein carbonyl as an indicator of oxidative damage in Drosophila melanogaster:: Influence of clock gene alleles and deficiencies in the formation of free-radical scavengers
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DOI：
10.1076/brhm.30.4.383.1414
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发表时间：
1999-10-01
影响因子：
1.1
通讯作者：
Hardeland, R
中科院分区：
文献类型：
--
作者：
Coto-Montes, A;Hardeland, R
在雄性黑腹果蝇中，蛋白质羰基被确定为自由基损伤的指标；选择该参数是因为与基于脂质过氧化的指标相比，它不受自由基反应链的自我维持进程的影响。昼夜时间模式是在野生型果蝇、Canton S品系和Canton S遗传背景下的以下突变体中确定的：短周期突变体per(s)；心律失常突变体 per(0)，同时由于无法表达羟基吲哚 O-甲基转移酶，因此缺乏褪黑激素的形成；突变体rosy (ry(506))，由于缺乏生理性自由基清除剂尿酸盐的形成，因此对氧化剂过敏。野生型果蝇表现出仅中等幅度的蛋白质羰基节律；仅在暗期中期发现蛋白质损伤增加。在per(s)中，LD 12:12中饲养的果蝇夜间蛋白质羰基最大值显着升高；此外，第二个极大值出现在光相的中间。心律失常突变体 per(0) 不表现出昼夜波动，甚至在 LD 中也不表现出。在尿酸缺失突变体 ry 中发现了最高浓度的蛋白质羰基。它的时间模式在很大程度上不同于其他菌株，在早晨和白天表现出最高的损害率。
In males of Drosophila melanogaster, protein carbonyl was determined as an indicator of damage by free radicals; this parameter was chosen because it is not affected by self-sustaining progression of radical reaction chains, in contrast to indicators based on lipid peroxidation. The diurnal time patterns were determined in wild-type flies, strain Canton S, and the following mutants, on a Canton S genetic background: the short-period mutant per(s); the arrhythmic mutant per(0), which is, at the same time, deficient for melatonin formation, due to its inability to express hydroxyindole O-methyltransferase; the mutant rosy (ry(506)), which is hypersensitive to oxidants because of its deficiency in the formation of the physiological free-radical scavenger urate. Wild-type flies exhibit a protein carbonyl rhythm of only moderate amplitude; an increase of protein damage is only found in the mid-scotophase. In per(s), the nocturnal protein carbonyl maximum of flies kept in LD 12:12 is markedly elevated; additionally, a secondary maximum appears in the middle of photophase. The arrhythmic mutant per(0) does not exhibit diurnal fluctuations, even not in LD. Highest concentrations of protein carbonyl are found in the urate-null mutant ry. Its temporal pattern largely deviates from the those of the other strains by showing highest rates of damage in the morning and during the day.