Fluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors.
Fluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors.
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荧光各向异性微孔板测定法研究全长类固醇受体辅激活剂-1a 与类固醇受体的相互作用。
DOI：
10.1007/978-1-62703-284-1_27
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Shapiro,DavidJ
中科院分区：
文献类型：
--
作者：
Zhang,Chen;Nordeen,StevenK;Shapiro,DavidJ
雌激素通过雌激素受体 (ER) 发挥作用，在生殖、中枢神经和骨骼系统的生长、分化和基因调控中发挥关键作用。 ER 介导的基因转录有助于乳腺癌、子宫癌和肝癌的发生和扩散。类固醇受体辅激活因子-1a (SRC1a) 属于 P160 共激活因子家族，是与 ER 介导的反式激活相关的众多共激活因子中最著名的。全长 P160 共激活剂与类固醇受体的结合很难在体外进行研究。本章详细介绍了如何使用荧光各向异性/偏振微孔板测定 (FAMA) 研究 SRC1a 与 ER 的相互作用。
Estrogens, acting via estrogen receptor (ER) play key roles in growth, differentiation, and gene regulation in the reproductive, central nervous, and skeletal systems. ER-mediated gene transcription contributes to the development and spread of breast, uterine, and liver cancer. Steroid receptor coactivator-1a (SRC1a) belongs to the P160 family of coactivators, which is the best known of the many coactivators implicated in ER-mediated transactivation. Binding of full-length P160 coactivators to steroid receptors has been difficult to investigate in vitro. This chapter details how to investigate the interaction of SRC1a with ER using the fluorescence anisotropy/polarization microplate assay (FAMA).