Nanoscale 3D Tracking with Conjugated Polymer Nanoparticles
Nanoscale 3D Tracking with Conjugated Polymer Nanoparticles
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DOI：
10.1021/ja907228q
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发表时间：
2009-12-30
影响因子：
15
通讯作者：
McNeill, Jason
中科院分区：
文献类型：
--
作者：
Yu, Jiangbo;Wu, Changfeng;McNeill, Jason
小的（类似于15 nm直径），高荧光共轭聚合物纳米粒子进行了评估，用于纳米级的2D和3D跟踪应用。由共轭聚合物组成的纳米粒子具有高吸收截面、高辐射速率和低或中等聚集猝灭，导致非常高的荧光亮度。明亮的荧光（类似于每20 ms曝光每个粒子检测到200 000个光子）产生小于1 nm的理论粒子跟踪不确定性。从固定和自由扩散粒子的轨迹分析确定了1-2 nm的横向跟踪不确定性。通过散焦成像获得用于3D颗粒跟踪的轴向（Z）位置信息。在固定的细胞中的单个颗粒的纳米级跟踪被证明，和一系列复杂的行为，可能是由于绑定/解绑定动力学，被观察到。
Small (similar to 15 nm diameter), highly fluorescent conjugated polymer nanoparticles were evaluated for nanoscale 2D and 3D tracking applications. Nanoparticles composed of conjugated polymers possess high absorption cross sections, high radiative rates, and low or moderate aggregation quenching, resulting in extraordinarily high fluorescent brightness. The bright fluorescence (similar to 200 000 photons detected per particle per 20 ms exposure) yields a theoretical particle tracking uncertainty of less than 1 nm. A lateral tracking uncertainty of 1-2 nm was determined from analysis of trajectories of fixed and freely diffusing particles. Axial (Z) position information for 3D particle tracking was obtained by defocused imaging. Nanoscale tracking of single particles in fixed cells was demonstrated, and a range of complex behaviors, possibly due to binding/unbinding dynamics, were observed.