Morphologic and functional evaluation of peripheral nerve fibers regenerated through polyimide sieve electrodes over long-term implantation
Morphologic and functional evaluation of peripheral nerve fibers regenerated through polyimide sieve electrodes over long-term implantation
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DOI：
10.1002/jbm.10099
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发表时间：
2002-06-15
期刊：
影响因子：
--
通讯作者：
Navarro, X
中科院分区：
文献类型：
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作者：
Ceballos, D;Valero-Cabré, A;Navarro, X
我们通过形态学和功能分析评估了聚酰亚胺再生型电极在长期植入过程中周围神经纤维的再生情况。将聚酰亚胺筛电极置于硅胶室内，植入大鼠坐骨神经断端之间，筛部分有281个直径为40μm的圆形通孔，通孔周围排列有9个集成记录刺激电极。在植入后 (mpi) 2、7 和 12 个月检查轴突再生程度。 13 只植入的动物中有 12 只成功再生。远端肌肉和神经的神经支配随着时间的推移而增加，在约 7 mpi 时达到稳定水平。有髓纤维的数量从 2 个月增加到 7 个月，此时与对照值相似。随着时间的推移，有髓纤维成熟，轴突直径和髓磷脂厚度显着增加。只有 0.6% 的再生轴突在植入电极附近显示出受压的迹象。大多数穿过通孔并通过远端神经再生的有髓纤维具有正常的外观。筛电极可用于植入后的神经刺激。通过不同的活性电极刺激神经束，引起不同形状和幅度的复合肌肉动作电位，表明选择性轴突刺激。 (C) 2002 年 Wiley 期刊公司
We evaluated by morphologic and functional analysis the regeneration of peripheral nerve fibers through polyimide regenerative-type electrodes over long-term implantation. Polyimide sieve electrodes were placed in silicone chambers and implanted between the severed ends of the sciatic nerve in rats, The sieve part had 281 round via holes of 40 mum in diameter, with nine integrated recording-stimulating electrodes arranged around the via holes. The degree of axonal regeneration was examined at 2, 7, and 12 months postimplantation (mpi). Regeneration was successful in 12 of the13 animals implanted. Reinnervation of distal muscle and nerves increased with time, reaching a plateau about 7 mpi. The number of myelinated fibers increased from 2 to 7 months, at which time it was similar to control values. With time the myelinated fibers matured, with significant increases in axon diameter and myelin thickness. Only 0.6% of the regenerated axons showed evidence of compression near the implanted electrode. The majority of the myelinated fibers that crossed the via holes and had been regenerated through the distal nerve had a normal appearance. Sieve electrodes were useful for nerve stimulation at postimplantation. Stimulation through different active electrodes excited nerve bundles, evoking compound muscle action potentials of varying shape and amplitude, indicative of selective axonal stimulation. (C) 2002 Wiley Periodicals, Inc.