Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
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DOI：
10.1083/jcb.202007052
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发表时间：
2021-01-04
期刊：
影响因子：
--
通讯作者：
Seemann J
中科院分区：
文献类型：
--
作者：
Zhang Y;Seemann J
By using a degron system, Zhang and Seemann find that acute depletion of GRASP55 and 65 does not affect Golgi stacking but indirectly compromises the integrity of the Golgi ribbon through loss of vesicle transport components. GRASP55 and GRASP65 have been implicated in stacking of Golgi cisternae and lateral linking of stacks within the Golgi ribbon. However, RNAi or gene knockout approaches to dissect their respective roles have often resulted in conflicting conclusions. Here, we gene-edited GRASP55 and/or GRASP65 with a degron tag in human fibroblasts, allowing for induced rapid degradation by the proteasome. We show that acute depletion of either GRASP55 or GRASP65 does not affect the Golgi ribbon, while chronic degradation of GRASP55 disrupts lateral connectivity of the ribbon. Acute double depletion of both GRASPs coincides with the loss of the vesicle tethering proteins GM130, p115, and Golgin-45 from the Golgi and compromises ribbon linking. Furthermore, GRASP55 and/or GRASP65 is not required for maintaining stacks or de novo assembly of stacked cisternae at the end of mitosis. These results demonstrate that both GRASPs are dispensable for Golgi stacking but are involved in maintaining the integrity of the Golgi ribbon together with GM130 and Golgin-45.
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