Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.
Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.
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支持正常和白血病造血细胞增殖和分化的肿瘤衍生生长因子。
DOI：
10.1111/j.1749-6632.1987.tb36235.x
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发表时间：
1987
影响因子：
5.2
通讯作者：
Rovera,G
中科院分区：
文献类型：
--
作者：
Tweardy,DJ;Caracciolo,D;Valtieri,M;Rovera,G
筛选了34种人肿瘤细胞系的条件培养基在体外骨髓培养物中诱导粒细胞-巨噬细胞集落、刺激小鼠IL-3依赖性造血细胞系（32 D克隆3）增殖以及刺激急性髓性白血病细胞悬浮培养物中胸苷掺入的能力。12个肿瘤细胞系产生的因子在这些试验中是活跃的。详细表征了胶质母细胞瘤细胞系U87 MG的条件培养基，发现其含有G-CSF和GM-CSF。U87 MG G-CSF的克隆和测序表明，它来源于G-CSF B mRNA，其编码在残基36-38处缺失3个氨基酸的蛋白质。G-CSF的基因被定位于人类染色体17带q21，这是一个在急性早幼粒细胞白血病中经常发现的易位区域。G-CSF（U87 MG）能够诱导小鼠IL-3依赖性细胞系32 D克隆3的总群体的粒细胞分化; IL-3可拮抗该作用。GM-CSF（U87-MG）支持两种生长因子依赖性白血病细胞系TALL 101和AML-193的增殖而不诱导分化。
The conditioned media of 34 human tumor cell lines were screened for the ability to induce granulocyte‐macrophage coloniesin vitroin bone marrow cultures, to stimulate proliferation of a murine IL‐3 dependent hemopoietic cell line (32D clone 3) and to stimulate thymidine incorporation in suspension cultures of acute myelogenous leukemia cells. Twelve tumor cell lines produced factors that were active in these assays. The conditioned medium of the glioblastoma cell line U87 MG was characterized in detail and found to contain G‐CSF and GM‐CSF. Cloning and sequencing of the U87 MG G‐CSF indicated that it was derived from G‐CSF b mRNA, which encodes a protein with a deletion of 3 amino acids at residues 36–38. The gene for G‐CSF was mapped to human chromosome 17 band q21, a region involved in translocations frequently found in acute promyelocytic leukemia. G‐CSF (U87MG) was able to induce granulocytic differentiation of the total population of a murine IL‐3 dependent cell line, 32D clone 3; this effect was antagonized by IL‐3. GM‐CSF (U87‐MG) supported the proliferation without inducing differentiation of two growth factor‐dependent leukemic cell lines, TALL 101 and AML‐193.