Conserved cytoplasmic domain sequences mediate the ER export of VZV, HSV-1, and HCMV gB.
Conserved cytoplasmic domain sequences mediate the ER export of VZV, HSV-1, and HCMV gB.
复制标题
保守的胞质结构域序列介导 VZV、HSV-1 和 HCMV gB 的 ER 输出。
DOI：
10.1016/j.virol.2004.07.011
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Assefa,Daniel
中科院分区：
文献类型：
--
作者：
Heineman,ThomasC;Connolly,Patrick;Hall,SusanL;Assefa,Daniel
糖蛋白B(GB)在疱疹病毒中保守，参与病毒侵入和细胞间传播。VZVgB的内质网输出是由两个胞质结构域AA818-826和C端17AA介导的，AA818-826含有YXXϕ基序。目前的研究检查了HSV-1和HCMV GB胞质区的相关序列是否类似地影响其GB同源物的ER输出。将定向突变引入HSV-1和HCMV GB的细胞质结构域，并测定突变蛋白获得高尔基依赖修饰的效率。HSV-1 GB和HCMV GB的正常ER输出都需要与VZV GB AA 818-826同源的序列。然而，HSV-1和HCMV gB的C末端区域对ER的输出没有影响。因此，α-和β-疱疹病毒gB同源物共享保守的ER输出信号，但gB的ER输出也存在物种特异性差异。
Glycoprotein B (gB) is conserved among the herpesviruses and participates in both virus entry and cell–cell spread. The ER export of VZV gB is mediated by two cytoplasmic domain regions, aa 818–826, which contains a YXXϕ motif, and the C-terminal 17 aa. The current study examines whether related sequences in the cytoplasmic domains of HSV-1 and HCMV gB similarly influence the ER export of their gB homologs. Directed mutations were introduced into the cytoplasmic domains of HSV-1 and HCMV gB, and the efficiencies with which the mutated proteins acquired Golgi-dependent modifications were determined. Sequences homologous to VZV gB aa 818–826 were required for normal ER export of both HSV-1 gB and HCMV gB. However, the C-terminal regions of HSV-1 and HCMV gB had no impact on ER export. Therefore, alpha- and betaherpesvirus gB homologs share conserved ER export signals, but species-specific differences in the ER export of gB also exist.