LFA-1 expression on target cells promotes human immunodeficiency virus type 1 infection and transmission
LFA-1 expression on target cells promotes human immunodeficiency virus type 1 infection and transmission
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DOI：
10.1128/jvi.75.2.1077-1082.2001
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
Tuen, M
中科院分区：
文献类型：
--
作者：
Hioe, CE;Chien, PC;Tuen, M
虽然CD4和趋化因子受体是人类免疫缺陷病毒(HN)的主要受体，但其他细胞蛋白，如LFA-1，也参与了HIV感染。LFA-1末端其配体ICAM-1、ICAM-2和ICAM-3可以表达在感染HIV的细胞上，也可以表达在HIV病毒粒子上。为了研究表达在靶细胞上的LFA-1在HIV感染中的作用，使用Jurkat来源的表达野生型LFA-1、成分活性突变体LFA-1或不表达LFA-1的J beta2.7 T细胞系，野生型LFA-1的存在增强了HIV感染的初始过程，以及随后的细胞间复制和传播。相比之下，具有结构性活性的LFA-1突变体未能促进病毒的复制和传播，尽管该突变体可以帮助艾滋病毒进入细胞并建立初始感染。这项研究清楚地证明了LFA-T在HIV感染的不同阶段的贡献。此外，LFA-1的表达不仅对最初的HN细胞相互作用、随后的复制和传播很重要，而且它的活性也必须受到适当的调节。
While CD4 and the chemokine receptors are the principal receptors for human immunodeficiency virus (HN), other cellular proteins, such as LFA-1, are also involved in HIV infection. LFA-1 end its ligands, ICAM-1, ICAM-2, and ICAM-3, can be expressed on the cells infected by HIV, as well as on the HIV virions themselves. To examine the role of LFA-1 expressed on target cells in HIV infection, Jurkat-derived J beta2.7 T-cell lines that express either wild-type LFA-1, a constitutively active mutant LFA-1, or no LFA-1 were used, The presence of wild-type LFA-1 enhanced the initial processes of HIV infection, as well as the subsequent replication and transmission from cell to cell. In contrast, the constitutively active LFA-1 mutant failed to promote virus replication and spread, even though this mutant could help HIV enter cells and establish the initial infection. This study clearly demonstrates the contribution of LFA-T in the different stages of HIV infection. Moreover, not only is LFA-1 expression important for initial HN-cell interaction, subsequent replication, and transmission, but its activity must also be properly regulated.