Dietary soya protein intake and exercise training have an additive effect on skeletal muscle fatty acid oxidation enzyme activities and mRNA levels in rats
Dietary soya protein intake and exercise training have an additive effect on skeletal muscle fatty acid oxidation enzyme activities and mRNA levels in rats
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DOI：
10.1079/bjn20061855
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发表时间：
2006-09-01
影响因子：
3.6
通讯作者：
Sugiura, Katsumi
中科院分区：
文献类型：
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作者：
Morifuji, Masashi;Sanbongi, Chiaki;Sugiura, Katsumi
运动训练和有规律的身体活动会增加脂肪的氧化。增强脂肪氧化对于预防高血压和肥胖等生活方式疾病很重要。本研究的目的是在大鼠中，以确定是否摄入膳食大豆蛋白和运动训练的活性和参与骨骼肌脂肪酸氧化的酶的mRNA表达的累加效应。将雄性Sprague-Dawley大鼠（n 32）随机分为4组（每组8只大鼠），然后进一步分为久坐组或运动训练组，喂食酪蛋白或大豆蛋白饲料。运动组大鼠进行为期2周的游泳训练，120 min/d，6 d/周。运动训练降低了肝脏三酰甘油水平和腹膜后脂肪组织重量，增加了骨骼肌肉毒碱棕榈酰转移酶1（CPT 1）活性和CPT 1、β-羟酰辅酶A脱氢酶（HAD）、酰基辅酶A氧化酶、过氧化物酶体增殖物激活物γ共激活因子1 α（PGC 1 α）和过氧化物酶体增殖物激活物激活物α的mRNA表达。大豆蛋白显著降低肝脏甘油三酯水平和附睾脂肪组织重量，增加骨骼肌CPT 1活性和CPT 1，HAD，酰基辅酶A氧化酶，中链酰基辅酶A脱氢酶，PGC 1 α和PPAR α mRNA水平。此外，骨骼肌HAD活性是最高的运动训练大鼠饲料大豆蛋白。我们的结论是，运动训练和大豆蛋白的摄入量有一个重要的添加剂的作用，诱导的过氧化物酶体增殖物激活受体途径，导致增加的活性和mRNA表达的酶参与骨骼肌中的脂肪酸氧化和减少体内脂肪的积累。
Exercise training and regular physical activity increase oxidation of fat. Enhanced oxidation of fat is important for preventing lifestyle diseases such as hypertension and obesity. The aim of the present study in rats was to determine whether intake of dietary soya protein and exercise training have an additive effect on the activity and mRNA expression of enzymes involved in skeletal muscle fatty acid oxidation. Male Sprague-Dawley rats (n 32) were assigned randomly into four groups (eight rats per group) and then divided further into sedentary or exercise-trained groups fed either casein or soya protein diets. Rats in the exercise groups were trained for 2 weeks by swimming for 120 min/d, 6 d/week. Exercise training decreased hepatic triacylglycerol levels and retroperitoneal adipose tissue weight and increased skeletal muscle carnitine palmitoyltransferase 1 (CPT1) activity and mRNA expression of CPT1, beta-hydroxyacyl-CoA dehydrogenase (HAD), acyl-CoA oxidase, PPAR gamma coactivator 1 alpha (PGC1 alpha) and PPAR alpha. Soya protein significantly decreased hepatic triacylglycerol levels and epididymal adipose tissue weight and increased skeletal muscle CPT1 activity and CPT1, HAD, acyl-CoA oxidase, medium-chain acyl-CoA dehydrogenase, PGC1 alpha and PPAR alpha mRNA levels. Furthermore, skeletal muscle HAD activity was the highest in exercise-trained rats fed soya protein. We conclude that exercise training and soya protein intake have an important additive role on induction of PPAR pathways, leading to increased activity and mRNA expression of enzymes involved in fatty acid oxidation in skeletal muscle and reduced accumulation of body fat.