The N-end rule pathway regulates pathogen responses in plants.
The N-end rule pathway regulates pathogen responses in plants.
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DOI：
10.1038/srep26020
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发表时间：
2016-05-13
影响因子：
4.6
通讯作者：
Graciet E
中科院分区：
文献类型：
--
作者：
de Marchi R;Sorel M;Mooney B;Fudal I;Goslin K;Kwaśniewska K;Ryan PT;Pfalz M;Kroymann J;Pollmann S;Feechan A;Wellmer F;Rivas S;Graciet E
为了有效地对抗病原体，植物依赖于一套复杂的免疫反应，这些免疫反应受到严格的调节，以允许防御程序的及时激活，适当的持续时间和足够的幅度。已知植物免疫反应的协调需要泛蛋白/蛋白酶体系统的活性，该系统控制真核生物中蛋白质的稳定性。在这里，我们证明了N-末端规则途径，泛素/蛋白酶体系统的一个子集，调节防御广泛的细菌和真菌病原体的模式植物拟南芥。我们表明，这一途径积极调节植物防御代谢产物，如芥子油苷的生物合成，以及生物合成和响应植物激素茉莉酸，这在植物免疫中起着关键作用。我们的研究结果还表明，N-末端规则途径的N-乙酰基化分支调节对模式病原体假单胞菌AvrRpm 1的防御程序的时间和幅度。
To efficiently counteract pathogens, plants rely on a complex set of immune responses that are tightly regulated to allow the timely activation, appropriate duration and adequate amplitude of defense programs. The coordination of the plant immune response is known to require the activity of the ubiquitin/proteasome system, which controls the stability of proteins in eukaryotes. Here, we demonstrate that the N-end rule pathway, a subset of the ubiquitin/proteasome system, regulates the defense against a wide range of bacterial and fungal pathogens in the model plant Arabidopsis thaliana. We show that this pathway positively regulates the biosynthesis of plant-defense metabolites such as glucosinolates, as well as the biosynthesis and response to the phytohormone jasmonic acid, which plays a key role in plant immunity. Our results also suggest that the arginylation branch of the N-end rule pathway regulates the timing and amplitude of the defense program against the model pathogen Pseudomonas syringae AvrRpm1.